Abstract | OBJECTIVES: This study was aimed to investigate effects of 2% minocycline-HCl paste root conditioning on periodontal surgery. MATERIALS AND METHODS: In vitro, cementum slices affected by periodontitis were randomly conditioned with 2% minocycline-HCl paste, 2% minocycline-HCl liquid, and 0.9% saline. NIH3T3 cells were cultured and attached to the each slide, and the viability and proliferation of cells were observed; In vivo, 21 deep periodontal pockets were treated by periodontal surgery, and the exposed root surfaces were randomly conditioned with 2% minocycline-HCl paste or 0.9% saline. The periodontal parameters were measured at baseline, after 3 and 6 months of periodontal surgery. RESULTS: In vitro, NIH3T3 cell showed better viability and proliferation at 3, 5, and 7 day in groups conditioned with minocycline-HCl than the group conditioned with 0.9% saline (at 3 day (P < 0.05); at 7 day (P < 0.01)) Minimal differences were found between minocycline-HCl paste and liquid groups; In vivo, 3 months after periodontal surgery, the greater CAL reduction was found in the minocycline-HCl treated group than in the control group (P < 0.05). The similar results were found for both CAL and PD (P < 0.05; P < 0.05) between two groups at 6 months after surgery. PI and SBI variations showed no statistical differences between two groups after periodontal surgery. CONCLUSION: Our results suggested that root conditioning with minocycline-HCl paste during periodontal surgery improve the periodontal healing, which may be associated with the promotion of the periodontal cell attachment and growth onto the root surfaces.
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Authors | Huihui Zhang, Xi Yang, Chengzhang Li, Shuhuan Shang, Jiaxi Wang |
Journal | International journal of clinical and experimental medicine
(Int J Clin Exp Med)
Vol. 8
Issue 3
Pg. 4080-6
( 2015)
ISSN: 1940-5901 [Print] United States |
PMID | 26064313
(Publication Type: Journal Article)
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