Evaluation of the oral toxicity of spinacine hydrochloride in a 13-week study in rats.

Abstract

Spinacine (4,5,6,7-tetrahydro-1H-imidazo[4,5-c]pyridine-6-carboxylic acid), the major reaction product of formaldehyde added to Grana Padano cheese, was administered to five groups of ten rats of each set at dietary concentrations that provided doses of O (control), 15, 30, 60 or 300 mg/kg body weight/day for 13 wk. There were no adverse effects upon survival, growth or food intake. No significant treatment-associated changes were found in haematological parameters, in serum chemistry or organ weights at the end of the study. At autopsy, neither gross nor histological modifications were attributable to treatment with spinacine. In this study the no-effect dose level for spinacine was considered to be 300 mg/kg body weight/day.

• Authors: C L Galli, P Allevi, D Colombo, E Corsini, P Marinelli, L Orlando, P Restani
• Journal: Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association (Food Chem Toxicol) Vol. 27 Issue 10 Pg. 651-6 (Oct 1989) ISSN: 0278-6915 [Print] England
• PMID: 2606401 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Food Additives
• Imidazoles
• Pyridines
• spinacine

Topics

• Administration, Oral
• Analysis of Variance
• Animals
• Blood Chemical Analysis
• Body Weight (drug effects)
• Cheese
• Female
• Food Additives (toxicity)
• Imidazoles (administration & dosage, toxicity)
• Male
• Organ Size (drug effects)
• Prothrombin Time
• Pyridines (administration & dosage, toxicity)
• Rats
• Rats, Inbred Strains