Effects of Wnt-10b on proliferation and differentiation of murine melanoma cells.

Abstract

In spite of the strong expression of Wnt-10b in melanomas, its role in melanoma cells has not been elucidated. In the present study, the biological effects of Wnt-10b on murine B16F10 (B16) melanoma cells were investigated using conditioned medium from Wnt-10b-producing COS cells (Wnt-CM). After 2 days of culture in the presence of Wnt-CM, proliferation of B16 melanoma cells was inhibited, whereas tyrosinase activity was increased. An in vitro wound healing assay demonstrated that migration of melanoma cells to the wound area was inhibited with the addition of Wnt-CM. Furthermore, evaluation of cellular senescence revealed prominent induction of SA-β-gal-positive senescent cells in cultures with Wnt-CM. Finally, the growth of B16 melanoma cell aggregates in collagen 3D-gel cultures was markedly suppressed in the presence of Wnt-CM. These results suggest that Wnt-10b represses tumor cell properties, such as proliferation and migration of B16 melanoma cells, driving them toward a more differentiated state along a melanocyte lineage.

• Authors: Masayasu Misu, Yukiteru Ouji, Norikazu Kawai, Fumihiko Nishimura, Fukumi Nakamura-Uchiyama, Masahide Yoshikawa
• Journal: Biochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 463 Issue 4 Pg. 618-23 (Aug 07 2015) ISSN: 1090-2104 [Electronic] United States
• PMID: 26056007 (Publication Type: Journal Article)
• Copyright: Copyright © 2015 Elsevier Inc. All rights reserved.

Chemical References

• Culture Media, Conditioned
• Wnt Proteins
• Wnt10b protein, mouse

Topics

• Animals
• Cell Differentiation (physiology)
• Cell Line, Tumor
• Cell Proliferation
• Cellular Senescence (physiology)
• Culture Media, Conditioned
• Male
• Melanoma, Experimental (pathology)
• Mice
• Mice, Inbred BALB C
• Wnt Proteins (physiology)