French guidelines recommend central intravenous (i.v.) infusion for high concentrations of
vancomycin, but peripheral intravenous (p.i.v.) infusion is often preferred in intensive care units.
Vancomycin infusion has been implicated in cases of
phlebitis, with endothelial toxicity depending on the drug concentration and the duration of the infusion.
Vancomycin is frequently infused in combination with other i.v.
antibiotics through the same administrative Y site, but the local toxicity of such combinations has been poorly evaluated. Such an assessment could improve
vancomycin infusion procedures in hospitals. Human umbilical vein endothelial cells (HUVEC) were challenged with clinical doses of
vancomycin over 24 h with or without other i.v.
antibiotics. Cell death was measured with the
alamarBlue test. We observed an excess cellular death rate without any synergistic effect but dependent on the numbers of combined infusions when
vancomycin and
erythromycin or
gentamicin were infused through the same Y site. Incompatibility between
vancomycin and
piperacillin-tazobactam was not observed in our study, and rinsing the cells between the two
antibiotic infusions did not reduce endothelial toxicity. No endothelial toxicity of
imipenem-cilastatin was observed when combined with
vancomycin. p.i.v.
vancomycin infusion in combination with other medications requires new recommendations to prevent
phlebitis, including limiting coinfusion on the same line, reducing the infusion rate, and choosing an intermittent infusion method. Further studies need to be carried out to explore other
drug combinations in long-term
vancomycin p.i.v.
therapy so as to gain insight into the mechanisms of drug incompatibility under multidrug infusion conditions.