Investigation of VIM, IMP, NDM-1, KPC AND OXA-48 enzymes in Enterobacteriaceae strains.

Abstract

Gram-negative bacteria especially Enterobacteriaceae species have become an increasing etiologic agent of nosocomial infections. The development of resistance to carbapenems have become an increasing problem in the treatment of nosocomial infections. Especially carbapenamases are common for Enterobacteriaceae strains. This study was performed to detect the types of carbapenemases in Enterobacteriaceae strains isolated from various clinical samples. Enterobacteriaceae species were isolated from urine, blood, tracheal aspirates, wound, and other respiratory samples. Susceptibility of isolates to imipenem, meropenem and ertapenem was tested. Carbapenemase genes were studied using HyplexSuperBug ID kit. VIM (1-13), IMP (1-22), NDM-1, KPC(1-10) and OXA-48 genes were investigated. Ninety-five isolates of Enterobacteriaceae spp. were included in the study. Sixty isolates were resistant to imipenem, meropenem and ertapenem and 20 isolates were found resistant to imipenem or ertapenem while 15 were susceptible to all carbapenems. Among the isolates with carbapenem resistance, 57 were positive for one carbapenemase gene and susceptible isolates did not have carbapenemase gene. OXA-48 was found in 49 of the isolates (86%), NDM-1 in 6 (10.5%) isolates, VIM in 2 isolates. IMP and KPC gene loci were not identified. Carbapenemase genes play a crucial role in the development and spread of resistant strains.

• Authors: Yelda Demir, Yasemin Zer, Ilkay Karaoglan
• Journal: Pakistan journal of pharmaceutical sciences (Pak J Pharm Sci) Vol. 28 Issue 3 Suppl Pg. 1127-33 (May 2015) ISSN: 1011-601X [Print] Pakistan
• PMID: 26051720 (Publication Type: Journal Article)

Chemical References

• Carbapenems
• Isoenzymes
• beta-Lactamases
• beta-lactamase NDM-1

Topics

• Carbapenems (metabolism, pharmacology)
• Drug Resistance, Multiple, Bacterial (genetics)
• Enterobacteriaceae (drug effects, enzymology, genetics)
• Enterobacteriaceae Infections (drug therapy, genetics, microbiology)
• Humans
• Hydrolysis
• Isoenzymes
• Microbial Sensitivity Tests
• Polymerase Chain Reaction
• Prospective Studies
• beta-Lactamases (classification, genetics, metabolism)