Abstract |
In the construction of artificial hip joint replacements, the surface and substrate of a cross-linked polyethylene (CLPE) liner are designed to achieve high wear resistance and prevent infection by bacteria. In this study, we fabricated a highly hydrophilic and antibiofouling poly(2-methacryloyloxyethyl phosphorylcholine [MPC]) (PMPC)-graft layer on the vitamin E-blended CLPE (HD-CLPE(VE)) surface. The 100-nm-thick, smooth, and electrically neutral PMPC layer was successfully fabricated on the HD-CLPE(VE) surface using photoinduced graft polymerization. The PMPC-grafted HD-CLPE(VE) was found to prevent bacterial adherence and biofilm formation on the surface because of the formation of a highly hydrophilic polyzwitterionic layer on the surface of HD-CLPE(VE), which can serve as an extremely efficient antibiofouling layer. The number of bacterial adhered on the PMPC-grafted HD-CLPE(VE) surface was reduced by 100-fold or more by PMPC grafting, regardless of the biofilm-production characteristics of the strains. In contrast, vitamin E blending did not affect bacterial adhesion. Moreover, the number of planktonic bacteria did not differ significantly, regardless of PMPC grafting and vitamin E blending. In conclusion, the PMPC-grafted HD-CLPE(VE) provided bacteriostatic effects associated with smooth, highly hydrophilic surfaces with a neutral electrostatic charge owing to the zwitterionic structure of the MPC unit. Thus, this modification may prove useful for the production of artificial hip joint replacement materials. STATEMENT OF SIGNIFICANCE: Our preliminary in vitro findings suggest that improved bacteriostatic performance of the HD-CLPE(VE) surface in orthopedic implants is possible via PMPC grafting. The results also indicate that surface modifications affect the anti- infection properties of the orthopedic implants and demonstrate that the application of a PMPC-grafted HD-CLPE(VE) surface may be a promising approach to extend the longevity and clinical outcomes of total hip arthroplasty. Further research is needed to evaluate the resistance to infection of PMPC-grafted HD-CLPE(VE) in terms of the varieties of biofilm formation tests including fluid flow conditions and animal experiments, which may offer useful clues to the possible performance of these materials in vivo.
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Authors | Masayuki Kyomoto, Takeo Shobuike, Toru Moro, Shihori Yamane, Yoshio Takatori, Sakae Tanaka, Hiroshi Miyamoto, Kazuhiko Ishihara |
Journal | Acta biomaterialia
(Acta Biomater)
Vol. 24
Pg. 24-34
(Sep 2015)
ISSN: 1878-7568 [Electronic] England |
PMID | 26050636
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2015 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved. |
Chemical References |
- Polymethacrylic Acids
- poly(2-methacryloyloxyethyl-phosphorylcholine)
- Phosphorylcholine
- Vitamin E
- Polyethylene
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Topics |
- Bacterial Adhesion
- Biofilms
(growth & development)
- Hip Prosthesis
(microbiology)
- Humans
- Phosphorylcholine
(analogs & derivatives, chemistry)
- Polyethylene
(chemistry)
- Polymethacrylic Acids
(chemistry)
- Staphylococcus aureus
(physiology)
- Vitamin E
(chemistry)
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