HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Tryptase and protease-activated receptor-2 stimulate scratching behavior in a murine model of ovalbumin-induced atopic-like dermatitis.

Abstract
The aim of the current study was to investigate the involvement of tryptase and protease-activated receptor-2 (PAR2) in the pathogenesis of itch using a recently developed murine model of atopic dermatitis (AD) elicited by epicutaneous sensitization with ovalbumin (OVA). We also examined whether tacrolimus exerts an antipruritic effect. Epicutaneous sensitization of BALB/c mice with OVA led to a significant increase in the number of scratches. Notably, PAR2 mRNA and protein levels as well as cutaneous levels of tryptase were significantly enhanced in epicutaneously sensitized mice. Pretreatment with the protease inhibitor, leupeptin, PAR2 antibody, and tacrolimus significantly reduced the number of degranulated mast cells and tryptase content, and consequently alleviated scratching behavior. Cetirizine (10mg/kg) exerted a significant inhibitory effect on the scratching behavior of mice, but did not affect the number of degranulated mast cells and induction of tryptase. Our results collectively suggest that tryptase and PAR2 are involved in OVA allergy-induced scratching behavior.
AuthorsYu Zhu, Wei Hua Pan, Xiao Rong Wang, Ying Liu, Min Chen, Xiao Guang Xu, Wan Qing Liao, Jin Hong Hu
JournalInternational immunopharmacology (Int Immunopharmacol) Vol. 28 Issue 1 Pg. 507-12 (Sep 2015) ISSN: 1878-1705 [Electronic] Netherlands
PMID26049029 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
CopyrightCopyright © 2015 Elsevier B.V. All rights reserved.
Chemical References
  • Allergens
  • RNA, Messenger
  • Receptor, PAR-2
  • Ovalbumin
  • Tryptases
Topics
  • Allergens
  • Animals
  • Behavior, Animal (drug effects)
  • Dermatitis, Atopic (metabolism, pathology)
  • Disease Models, Animal
  • Female
  • Mice, Inbred BALB C
  • Ovalbumin
  • Pruritus (metabolism, pathology)
  • RNA, Messenger (metabolism)
  • Receptor, PAR-2 (genetics, metabolism)
  • Skin (drug effects, metabolism, pathology)
  • Tryptases (metabolism)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: