Development of co-dominant SCAR markers linked to resistant gene against the Fusarium oxysporum f. sp. radicis-lycopersici.

Abstract	We developed highly reliable co-dominant SCAR markers linked to the Frl gene. FORL testing is difficult. The marker is expected to be quickly adapted for MAS by tomato breeders. Fusarium oxysporum f. sp. radicis-lycopersici causes Fusarium crown and root rot (FCR), an economically important soil-borne disease of tomato. The resistance against FCR is conferred by a single dominant gene (Frl) located on chromosome 9. The aim of this study was to develop molecular markers linked to the Frl gene for use in marker-assisted breeding (MAS) programs. The FCR-resistant 'Fla. 7781' and susceptible 'B560' lines were crossed, and F1 was both selfed and backcrossed to 'B560' to generate segregating F2 and BC1 populations. The two conserved set II (COSII) markers were found linked to the Frl gene, one co-segregated with FCR resistance in both F2 and BC1 populations and the other was 8.5 cM away. Both COSII markers were converted into co-dominant SCAR markers. SCARFrl marker produced a 950 and a 1000 bp fragments for resistant and susceptible alleles, respectively. The linkage of SCARFrl marker was confirmed in BC2F3 populations developed by backcrossing the resistant 'Fla. 7781' to five different susceptible lines. The SCARFrl marker has been in use in the tomato breeding programs in BATEM, Antalya, Turkey, since 2012 and has proved highly reliable. The SCARFrl marker is expected to aid in the development of FCR-resistant lines via marker-assisted selection (MAS).
Authors	Nedim Mutlu, Aylin Demirelli, Hülya Ilbi, Cengiz Ikten
Journal	TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik (Theor Appl Genet) Vol. 128 Issue 9 Pg. 1791-8 (Sep 2015) ISSN: 1432-2242 [Electronic] Germany
PMID	26037087 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References	DNA, Plant Genetic Markers
Topics	DNA, Plant (genetics) Disease Resistance (genetics) Fusarium Genes, Dominant Genes, Plant Genetic Linkage Genetic Markers Solanum lycopersicum (genetics, microbiology) Plant Breeding Plant Diseases (genetics) Sequence Analysis, DNA

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