SDF-1/CXCR4 promotes F5M2 osteosarcoma cell migration by activating the Wnt/β-catenin signaling pathway.
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| Abstract |
Osteosarcoma (OS), the most common primary malignant bone tumor in children and adolescents, lacks an effective therapy. Stromal cell-derived factor (SDF-1) and its receptor, CXCR4, play multiple roles in migration, proliferation, and survival of different tumor cells. This study aimed to investigate whether the functional SDF-1/CXCR4 signaling mediates chemotaxis in F5M2 OS cells as well as the underlying mechanisms. Immunohistochemistry and immunofluorescence microscopy were used. RNA expression was detected by real-time quantitative polymerase chain reaction, and protein expression was examined by Western blotting. Migration assays were carried out in F5M2 cells. The results showed that the expression of CXCR4 and β-catenin mRNA and protein was significantly higher in OS tissues compared to the surrounding non-neoplastic tissues. SDF-1 promoted F5M2 cell migration by activating the AKT and Wnt/β-catenin signaling pathway, which was abrogated by preincubation with AMD3100 and LY294002. In conclusion, SDF-1/CXCR4 axis-promoted F5M2 cell migration was regulated by the Wnt/β-catenin signaling pathway.
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| Authors | Yao Lu, Bin Hu, Guo-Feng Guan, Jie Chen, Chun-qiu Wang, Qiong Ma, Yan-Hua Wen, Xiu-Chun Qiu, Xiao-ping Zhang, Yong Zhou |
| Journal | Medical oncology (Northwood, London, England) (Med Oncol) Vol. 32 Issue 7 Pg. 194 (Jul 2015) ISSN: 1559-131X [Electronic] United States |
| PMID | 26026718 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't) |
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