Delivery of human NKG2D-IL-15 fusion gene by chitosan nanoparticles to enhance antitumor immunity.

Abstract

Nanoparticles are becoming promising carriers for gene delivery because of their high capacity in gene loading and low cell cytotoxicity. In this study, a chitosan-based nanoparticle encapsulated within a recombinant pcDNA3.1-dsNKG2D-IL-15 plasmid was generated. The fused dsNKG2D-IL-15 gene fragment consisted of double extracellular domains of NKG2D with IL-15 gene at downstream. The average diameter of the gene nanoparticles ranged from 200 nm to 400 nm, with mean zeta potential value of 53.8 ± 6.56 mV. The nanoparticles which were loaded with the dsNKG2D-IL-15 gene were uptaken by tumor cells with low cytotoxicity. Tumor cells pre-transfected by gene nanopartilces stimulated NK and T cells in vitro. Intramuscular injection of gene nanoparticles suppressed tumor growth and prolonged survival of tumor-bearing mice through activation of NK and CD8(+) T cells. Thus, chitosan-based nanoparticle delivery of dsNKG2D-IL-15 gene vaccine can be potentially used for tumor therapy.

• Authors: Chen Yan, Leng Jie, Wang Yongqi, Xiao Weiming, Xi Juqun, Ding Yanbing, Qian Li, Pan Xingyuan, Ji Mingchun, Gong Weijuan
• Journal: Biochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 463 Issue 3 Pg. 336-43 (Jul 31 2015) ISSN: 1090-2104 [Electronic] United States
• PMID: 26022121 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2015 Elsevier Inc. All rights reserved.

Chemical References

• Cancer Vaccines
• Interleukin-15
• KLRK1 protein, human
• NK Cell Lectin-Like Receptor Subfamily K
• Vaccines, DNA
• Chitosan

Topics

• Animals
• Cancer Vaccines (administration & dosage, immunology, therapeutic use)
• Cell Line
• Cell Line, Tumor
• Chitosan (chemistry)
• Humans
• Immunity
• Interleukin-15 (genetics)
• Melanoma (immunology, pathology, prevention & control)
• Mice, Inbred C57BL
• NK Cell Lectin-Like Receptor Subfamily K (genetics)
• Nanoparticles (chemistry, ultrastructure)
• Plasmids (administration & dosage, immunology, therapeutic use)
• Transfection
• Vaccines, DNA (administration & dosage, immunology, therapeutic use)