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[Ganoderic acid A suppresses proliferation and invasion and induces apoptosis in human osteosarcoma cells].

AbstractOBJECTIVE:
To investigate the effect of ganoderic acid A (GA-A) on the biological behaviors of human osteosarcoma cells in vitro.
METHODS:
MG63 and HOS cells were treated with 0.1, 0.25, and 0.5 mmol/L GA-A, and the changes in cell proliferation, apoptosis and migration were evaluated using MTT assay, flow cytometry, and Transwell assay, respectively. The expressions of STAT3, p38, and NF-κB1 in the cells were analyzed by Western blotting.
RESULTS:
GA-A effectively inhibited the proliferation of human osteosarcoma HOS and MG-63 cells in a dose-dependent manner, and induced obvious cell apoptosis in both cells. Treatment with 0.5 mmol/L GA-A also resulted in significant inhibition of the invasion of both cells. The results of Western blotting showed that GA-A down-regulated the expression level of phosphorylated STAT3 and increased the phosphorylation level of p38 and NF-κB1 expression in both cells.
CONCLUSION:
GA-A can induce proliferation inhibition, apoptosis and suppression of invasion in human osteosarcoma HOS and MG-63 cells.
AuthorsJianli Shao, Zhizhong Li, Genlong Jiao, Guodong Sun, Zhigang Zhou
JournalNan fang yi ke da xue xue bao = Journal of Southern Medical University (Nan Fang Yi Ke Da Xue Xue Bao) Vol. 35 Issue 5 Pg. 619-24 (May 2015) ISSN: 2663-0842 [Electronic] China
PMID26018252 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Heptanoic Acids
  • NF-kappa B p50 Subunit
  • NFKB1 protein, human
  • STAT3 Transcription Factor
  • STAT3 protein, human
  • Lanosterol
  • ganoderic acid A
  • p38 Mitogen-Activated Protein Kinases
Topics
  • Apoptosis (drug effects)
  • Bone Neoplasms (pathology)
  • Cell Line, Tumor (drug effects)
  • Cell Movement (drug effects)
  • Cell Proliferation (drug effects)
  • Heptanoic Acids (pharmacology)
  • Humans
  • Lanosterol (analogs & derivatives, pharmacology)
  • NF-kappa B p50 Subunit (metabolism)
  • Osteosarcoma (pathology)
  • Phosphorylation
  • STAT3 Transcription Factor (metabolism)
  • p38 Mitogen-Activated Protein Kinases (metabolism)

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