Tendon regeneration with a novel tendon hydrogel: in vitro effects of platelet-rich plasma on rat adipose-derived stem cells.

Abstract

BACKGROUND:

Tendon hydrogel is a promising new injectable substance that has been shown to improve repair strength after tendon injury. This study assesses the capacity of platelet-rich plasma to stimulate proliferation and migration of rat adipose-derived stem cells in tendon hydrogel in vitro.

METHODS:

To assess proliferation, adipose-derived stem cells were exposed to plasma, plasma supplemented with growth factors, or platelet-rich plasma in culture medium and tendon hydrogel. To assess migration, adipose-derived stem cells were plated onto tendon hydrogel -coated wells and covered with medium containing plasma, plasma supplemented with growth factors, platelet-rich plasma, or bovine serum albumin. Migration from cell-seeded to cell-free zones was assessed at 12-hour intervals.

RESULTS:

Platelet-rich plasma augmented proliferation to a greater extent compared with plasma and plasma supplemented with growth factors (10%: optical density, 1.18 versus 0.75 versus 0.98, respectively). Platelet-rich plasma was superior to plasma in tendon hydrogel (10%: optical density, 1.19 versus 0.85) but did not augment proliferation to the extent that plasma supplemented with growth factors did (10%: optical density, 1.19 versus 1.56). Platelet-rich plasma enhanced the migration of adipose-derived stem cells compared with serum-free medium (bovine serum albumin) (36 hours: platelet-rich plasma, 1.88; plasma, 1.51; plasma plus growth factor, 1.80; bovine serum albumin, 1.43).

CONCLUSIONS:

Tendon healing is mediated by migration of cells to the injured area and cellular proliferation at that site. Tendon hydrogel supplemented with platelet-rich plasma stimulates these processes. Future studies will evaluate this combination's ability to stimulate healing in chronic tendon injuries in vivo.

• Authors: Christopher S Crowe, Grace Chiou, Rory McGoldrick, Kenneth Hui, Hung Pham, James Chang
• Journal: Plastic and reconstructive surgery (Plast Reconstr Surg) Vol. 135 Issue 6 Pg. 981e-989e (Jun 2015) ISSN: 1529-4242 [Electronic] United States
• PMID: 26017614 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Culture Media
• Hydrogel, Polyethylene Glycol Dimethacrylate

Topics

• Adipocytes (transplantation)
• Animals
• Cell Proliferation (drug effects)
• Cells, Cultured
• Culture Media
• Hydrogel, Polyethylene Glycol Dimethacrylate (pharmacology)
• In Vitro Techniques
• Models, Animal
• Platelet-Rich Plasma
• Random Allocation
• Rats
• Rats, Sprague-Dawley
• Reference Values
• Regeneration (physiology)
• Sensitivity and Specificity
• Stem Cells (cytology, physiology)
• Tendons (physiology, surgery)