Abstract | PURPOSE: METHODS: Thirty-six PTCs were divided into two groups according to the 2009 American Thyroid Association risk classification (17 low, 19 intermediate), and each group was divided into subgroups based on the presence or absence of the BRAFV600E mutation (21 BRAF mutated, 15 BRAF wild type). Gene expression was analyzed using fluidic cards containing probes and primers specific for the THRβ gene, as well as for genes of thyroperoxidase (TPO), sodium/iodide symporter (NIS), thyroglobulin (Tg) and thyroid stimulating hormone receptor (TSH-R) and for some miRNAs involved in thyroid neoplasia and targeting THRβ. The mRNA levels of each tumor tissue were compared with their correspondent normal counterpart. RESULTS: THRβ transcript was down-regulated in all PTCs examined. No significant differences were found between intermediate- vs low-risk PTCs patients, and BRAF-mutated vs BRAF wild-type groups. THRβ expression was directly correlated with NIS, TPO, Tg and TSH-R, and inversely correlated to miR-21, -146a, -181a and -221 expression. CONCLUSIONS: Our results demonstrate that down-regulation of THRβ is a common feature of PTCs. While it is not associated with a more aggressive phenotype of PTC, it correlates with the reduction of all the markers of differentiation and is associated with overexpression of some miRNAs supposed to play a role in thyroid tumorigenesis.
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Authors | F Rosignolo, V Maggisano, M Sponziello, M Celano, C R T Di Gioia, M D'Agostino, L Giacomelli, A Verrienti, M Dima, V Pecce, C Durante |
Journal | Journal of endocrinological investigation
(J Endocrinol Invest)
Vol. 38
Issue 12
Pg. 1283-9
(Dec 2015)
ISSN: 1720-8386 [Electronic] Italy |
PMID | 26003825
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Biomarkers, Tumor
- MicroRNAs
- RNA, Messenger
- Thyroid Hormone Receptors beta
- BRAF protein, human
- Proto-Oncogene Proteins B-raf
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Topics |
- Adult
- Aged
- Biomarkers, Tumor
(metabolism)
- Carcinoma
(genetics, metabolism)
- Carcinoma, Papillary
- Down-Regulation
- Female
- Gene Expression
- Humans
- Male
- MicroRNAs
(metabolism)
- Middle Aged
- Proto-Oncogene Proteins B-raf
(genetics)
- RNA, Messenger
(metabolism)
- Thyroid Cancer, Papillary
- Thyroid Hormone Receptors beta
(genetics, metabolism)
- Thyroid Neoplasms
(genetics, metabolism)
- Young Adult
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