Proteomics analysis of human oligodendroglioma proteome.

Abstract

Proteomics analyses enable the identification and quantitation of proteins. From a purely clinical perspective, the application of proteomics based on innovations, may greatly affect the future management of malignant brain tumors. This optimism is based on four main reasons: diagnosis, prognosis, selection of targeted therapy based on molecular profile of the brain tumor and monitoring therapeutic response, or resistance. We extracted the proteins of tumor and normal brain tissues, and then evaluated the protein purity by Bradford test. In this study, we separated the proteins by two-dimensional (2DG) gel electrophoresis methods. Then spots were analyzed, compared using statistical data and specific software and were identified by pH isoelectric, molecular weights and data banks. The protein profiles were determined using 2D gel electrophoresis and MALDI TOF/TOF mass spectrometry approaches. Simple statistical tests were used to establish a putative hierarchy in which the change in protein level was ranked according to a cut-off point with p<0.05. The 2D gel showed a total of 1328 spots among which 157 spots were under-expressed and 276 spots were overexpressed. Most proteins are subjects to post-translational modifications, where amino acid residues may be chemically modified or conjugated by small proteins like ubiquitin. Proteomics is a powerful way to identifying multiple proteins which are altered following a neuropharmacological intervention in a CNS disease.

• Authors: Solmaz Khaghani-Razi-Abad, Mehrdad Hashemi, Mehdi Pooladi, Maliheh Entezari, Elham Kazemi
• Journal: Gene (Gene) Vol. 569 Issue 1 Pg. 77-82 (Sep 10 2015) ISSN: 1879-0038 [Electronic] Netherlands
• PMID: 26002447 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2015 Elsevier B.V. All rights reserved.

Topics

• Adult
• Aged
• Brain Neoplasms (genetics, pathology)
• Electrophoresis, Gel, Two-Dimensional
• Female
• Gene Expression Regulation, Neoplastic
• Humans
• Male
• Middle Aged
• Oligodendroglioma (genetics, pathology)
• Proteomics