Chromosomal rearrangements involving the NTRK1 gene in colorectal carcinoma.

Abstract

Chromosomal rearrangements of the NTRK1 gene, which encodes the high affinity nerve growth factor receptor (tropomyosin related kinase, TRKA), have been observed in several epithelial cancers, such as colon cancer, papillary thyroid carcinoma or non small cell lung cancer. The various NTRK1 fusions described so far lead to constitutive activation of TRKA kinase activity and are oncogenic. We further investigated here the existence and the frequency of NTRK1 gene rearrangements in colorectal cancer. Using immunohistochemistry and quantitative reverse transcriptase PCR, we analyzed a series of human colorectal cancers. We identified two TRKA positive cases over 408, with NTRK1 chromosomal rearrangements. One of these rearrangements is a TPM3-NTRK1 fusion already observed in colon cancer, while the second one is a TPR-NTRK1 fusion never described in this type of cancer. These findings further confirm that translocations in the NTRK1 gene are recurring events in colorectal cancer, although occurring at a low frequency (around 0.5%).

• Authors: Laurent Créancier, Isabelle Vandenberghe, Bruno Gomes, Caroline Dejean, Jean-Christophe Blanchet, Julie Meilleroux, Rosine Guimbaud, Janick Selves, Anna Kruczynski
• Journal: Cancer letters (Cancer Lett) Vol. 365 Issue 1 Pg. 107-11 (Aug 28 2015) ISSN: 1872-7980 [Electronic] Ireland
• PMID: 26001971 (Publication Type: Journal Article)
• Copyright: Copyright © 2015 Elsevier Ireland Ltd. All rights reserved.

Chemical References

• Biomarkers, Tumor
• Nuclear Pore Complex Proteins
• Proto-Oncogene Proteins
• TPM3 protein, human
• TPR protein, human
• Tropomyosin
• Receptor, trkA

Topics

• Animals
• Base Sequence
• Biomarkers, Tumor (analysis, genetics)
• Carcinoma (chemistry, genetics, pathology)
• Cell Line, Tumor
• Colorectal Neoplasms (chemistry, genetics, pathology)
• Gene Fusion
• Gene Rearrangement
• Humans
• Immunohistochemistry
• Mice, Nude
• Molecular Sequence Data
• Nuclear Pore Complex Proteins (genetics)
• Proto-Oncogene Proteins (genetics)
• Receptor, trkA (analysis, genetics)
• Reverse Transcriptase Polymerase Chain Reaction
• Sequence Analysis, DNA
• Tissue Array Analysis
• Translocation, Genetic
• Tropomyosin (genetics)