Abstract | BACKGROUND: METHODS: Nipple discharge samples were collected from three intraductal carcinoma breast cancer patients and three intraductal papilloma patients. The initial screening of miRNA expression was performed with an Axon GenePix 4000B microarray scanner using a novel approach to label miRNAs. The expression levels of the miRNAs selected from the initial screening were further examined by quantitative real-time polymerase chain reaction (qRT-PCR) in 21 validation samples (8 carcinomas and 13 benign tumors). An independent t test was used to detect significant correlations between the miRNA expression levels and breast cancer. RESULTS: CONCLUSION: The novel approach of using a microarray scanner is applicable for studying biomarkers in nipple discharge containing small amounts of miRNA. miRNAs could serve as potential tumor biomarkers that can assist in breast cancer screening. Up-regulation of miR-4484, miR-K12-5-5p, and miR-3646 in nipple discharge may be a predictor of malignant breast cancer.
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Authors | Kai Zhang, Song Zhao, Qing Wang, Hsin-Sheng Yang, Jiang Zhu, Rong Ma |
Journal | Annals of surgical oncology
(Ann Surg Oncol)
Vol. 22 Suppl 3
Pg. S536-44
(Dec 2015)
ISSN: 1534-4681 [Electronic] United States |
PMID | 25976861
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
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Chemical References |
- Biomarkers, Tumor
- MicroRNAs
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Topics |
- Biomarkers, Tumor
(genetics)
- Breast Neoplasms
(diagnosis, genetics, metabolism)
- Carcinoma, Intraductal, Noninfiltrating
(diagnosis, genetics, metabolism)
- Early Detection of Cancer
- Female
- Follow-Up Studies
- Gene Expression Profiling
- Gene Expression Regulation, Neoplastic
- Humans
- MicroRNAs
(genetics)
- Neoplasm Invasiveness
- Neoplasm Staging
- Nipples
(metabolism)
- Oligonucleotide Array Sequence Analysis
- Papilloma
(diagnosis, genetics, metabolism)
- Prognosis
- Real-Time Polymerase Chain Reaction
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