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Bax inhibitor-1 is overexpressed in non-small cell lung cancer and promotes its progression and metastasis.

Abstract
Bax inhibitor-1 (BI-1) is a novel anti-apoptotic protein. While the effect of BI-1 on apoptosis has been extensively studied, less is known about how BI-1 is related to oncogenesis and the progression, particularly, the invasion and metastasis of cancer. In this study, we investigated the expression and function of BI-1 in human non-small cell lung cancer (NSCLC). RT-PCR and immunohistochemistry were performed on clinical NSCLC samples to detect BI-1 expression. Northern blot and Western blot analysis were performed to detect BI-1 expression of in NSCLC cell lines. Cell proliferation and apoptosis were analyzed by flow cytometry. Our results showed that the overexpression of BI-1 was significantly related to oncogenesis of NSCLC (P < 0.05). Meanwhile, we identified a novel 5'end from normal lung-derived BI-1 transcript, different from any transcript deposit in the Genbank, but we detected no mutations in the coding sequence and the promoter region of BI-1 and no abnormal splicing of the alternative first exon. Ectopic expression of BI-1 changed the proliferation and apoptosis of AGZY83-a and Anip973 cells. In conclusion, BI-1 is overexpressed in NSCLC and promotes the progression and metastasis of NSCLC.
AuthorsBingjie Lu, Yu Li, Hua Li, Yingmei Zhang, Jin Xu, Lihong Ren, Songbin Fu, Yi Zhou
JournalInternational journal of clinical and experimental pathology (Int J Clin Exp Pathol) Vol. 8 Issue 2 Pg. 1411-8 ( 2015) ISSN: 1936-2625 [Electronic] United States
PMID25973025 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Apoptosis Regulatory Proteins
  • Biomarkers, Tumor
  • Membrane Proteins
  • TMBIM6 protein, human
Topics
  • Adult
  • Apoptosis (physiology)
  • Apoptosis Regulatory Proteins (analysis, biosynthesis)
  • Biomarkers, Tumor (analysis)
  • Carcinoma, Non-Small-Cell Lung (pathology)
  • Cell Line, Tumor
  • Cell Proliferation
  • Disease Progression
  • Flow Cytometry
  • Humans
  • Immunoblotting
  • Immunohistochemistry
  • Lung Neoplasms (pathology)
  • Male
  • Membrane Proteins (analysis, biosynthesis)
  • Neoplasm Invasiveness (pathology)
  • Reverse Transcriptase Polymerase Chain Reaction

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