Abstract |
Bax inhibitor-1 (BI-1) is a novel anti-apoptotic protein. While the effect of BI-1 on apoptosis has been extensively studied, less is known about how BI-1 is related to oncogenesis and the progression, particularly, the invasion and metastasis of cancer. In this study, we investigated the expression and function of BI-1 in human non-small cell lung cancer (NSCLC). RT-PCR and immunohistochemistry were performed on clinical NSCLC samples to detect BI-1 expression. Northern blot and Western blot analysis were performed to detect BI-1 expression of in NSCLC cell lines. Cell proliferation and apoptosis were analyzed by flow cytometry. Our results showed that the overexpression of BI-1 was significantly related to oncogenesis of NSCLC (P < 0.05). Meanwhile, we identified a novel 5'end from normal lung-derived BI-1 transcript, different from any transcript deposit in the Genbank, but we detected no mutations in the coding sequence and the promoter region of BI-1 and no abnormal splicing of the alternative first exon. Ectopic expression of BI-1 changed the proliferation and apoptosis of AGZY83-a and Anip973 cells. In conclusion, BI-1 is overexpressed in NSCLC and promotes the progression and metastasis of NSCLC.
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Authors | Bingjie Lu, Yu Li, Hua Li, Yingmei Zhang, Jin Xu, Lihong Ren, Songbin Fu, Yi Zhou |
Journal | International journal of clinical and experimental pathology
(Int J Clin Exp Pathol)
Vol. 8
Issue 2
Pg. 1411-8
( 2015)
ISSN: 1936-2625 [Electronic] United States |
PMID | 25973025
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Apoptosis Regulatory Proteins
- Biomarkers, Tumor
- Membrane Proteins
- TMBIM6 protein, human
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Topics |
- Adult
- Apoptosis
(physiology)
- Apoptosis Regulatory Proteins
(analysis, biosynthesis)
- Biomarkers, Tumor
(analysis)
- Carcinoma, Non-Small-Cell Lung
(pathology)
- Cell Line, Tumor
- Cell Proliferation
- Disease Progression
- Flow Cytometry
- Humans
- Immunoblotting
- Immunohistochemistry
- Lung Neoplasms
(pathology)
- Male
- Membrane Proteins
(analysis, biosynthesis)
- Neoplasm Invasiveness
(pathology)
- Reverse Transcriptase Polymerase Chain Reaction
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