HOMEPRODUCTSSERVICESCOMPANYCONTACTFAQResearchDictionaryPharmaMobileSign Up FREE or Login

Sensitivity of neoplastic cells to senescence unveiled under standard cell culture conditions.

AbstractBACKGROUND:
Cancer cells are typically defined as infinitely proliferating, whereas normal cells (except stem cells) are considered as being programmed to become senescent. Our data show that this characterization is misleading.
MATERIALS AND METHODS:
Multiplex Ligation-dependent Probe Amplification, TP53 sequencing, real-time polymerase chain reaction (PCR) for MUC1 and SCGB2A2 and immunocytochemistry, together with senescence detection assay and real-time microscopic observations were used to analyze primary neoplastic cells isolated from prostate, breast and colorectal tumors, as well as stable cancer cell lines (MCF7, MDA-MB-468, SW962, SK-MEL28, NCI-H1975 and NCI-H469).
RESULTS:
In all cases of primary cancer cell cultures, in vitro conditions rapidly revealed senescence in the majority of cells. Two out of six stable cancer cell lines did not exhibit any senescence-associated-β-Galactosidase-positive cells. Interestingly, four cell lines had small sub-populations of senescent cells (single SA-β-Gal-positive cells).
CONCLUSION:
Primary neoplastic cells from different types of cancer (prostate, breast, colon cancer) appear to be senescent in vitro. Apparently, cancer cell lines that have been used for many years in drug-testing analyses have constantly been misleading researchers in terms of the general sensitivity of cancer cells to senescence.
AuthorsJolanta Zieba, Magdalena Ksiazkiewcz, Karolina Janik, Mateusz Banaszczyk, Joanna Peciak, Sylwester Piaskowski, Marek Lipinski, Michal Olczak, Ewelina Stoczynska-Fidelus, Piotr Rieske
JournalAnticancer research (Anticancer Res) Vol. 35 Issue 5 Pg. 2759-68 (May 2015) ISSN: 1791-7530 [Electronic] Greece
PMID25964555 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
CopyrightCopyright© 2015 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved.
Chemical References
  • MUC1 protein, human
  • Mammaglobin A
  • Mucin-1
  • SCGB2A2 protein, human
Topics
  • Breast Neoplasms (drug therapy, genetics, pathology)
  • Cell Aging (drug effects, genetics)
  • Colonic Neoplasms (drug therapy, genetics, pathology)
  • Female
  • Humans
  • MCF-7 Cells
  • Male
  • Mammaglobin A (biosynthesis)
  • Mucin-1 (biosynthesis)
  • Prostatic Neoplasms (drug therapy, genetics, pathology)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research network!


Choose Username:
Email:
Password:
Verify Password: