Transplantation of human amnion mesenchymal cells attenuates the disease development in rats with collagen-induced arthritis.

Abstract

OBJECTIVES:

Human amnion mesenchymal cells (hAMCs), isolated from the amniotic membrane of human placenta, are a unique population of mesenchymal stem cells (MSCs). Recent studies indicated that hAMCs had immunosuppressive functions and might be used in treatment of some autoimmune diseases. The aim of this study is to explore the feasibility of using hAMCs for treatment rats with collagen-induced arthritis (CIA), a classic animal model for human rheumatoid arthritis.

METHODS:

SD rats were immunised with type II collagen and Freund's incomplete adjuvant. hAMCs were injected intraperitoneal when arthritis had become established. The arthritis was evaluated macroscopically and microscopically. Serum levels of IFN-γ, TNF-α, SOD, MDA, GSH-Px and T-AOC were detected by commercially assay kits. CD4⁺/CD8⁺ T-cell ratio in peripheral blood was examined by flow cytometry. Proliferation of splenocytes was evaluated using MTT assay.

RESULTS:

The results demonstrated that application of hAMCs significantly ameliorated severity of arthritis and decreased the histopathological changes in CIA rats. Consistently, production of proinflammatory cytokines such as IFN-γ and TNF-α was dramatically inhibited. Moreover, hAMCs exerted anti-oxidative capacity by significantly raising the levels of SOD, GSH-Px, T-AOC and lowering the level of MDA. In addition, hAMCs also remarkably restored CD4⁺/CD8⁺ T-cell ratio and induced hyporesponsiveness of T lymphocytes by inhibiting their active proliferation. Finally, hAMCs had no obvious side effect on CIA rats.

CONCLUSIONS:

In conclusion, our results indicated that hAMCs could attenuate the disease development in rats with CIA, which might be a promising cell source for therapy of rheumatoid arthritis.

• Authors: Jun Shu, Lin Pan, Xiaojie Huang, Ping Wang, Hong Li, Xiaojuan He, Zhe Cai
• Journal: Clinical and experimental rheumatology (Clin Exp Rheumatol) 2015 Jul-Aug Vol. 33 Issue 4 Pg. 484-90 ISSN: 0392-856X [Print] Italy
• PMID: 25962385 (Publication Type: Journal Article)

Chemical References

• Adjuvants, Immunologic
• Lipids
• Tumor Necrosis Factor-alpha
• incomplete Freund's adjuvant
• Interferon-gamma
• Freund's Adjuvant

Topics

• Adjuvants, Immunologic (administration & dosage)
• Amnion (pathology)
• Animals
• Arthritis, Experimental (blood, pathology, therapy)
• Arthritis, Rheumatoid (therapy)
• Drug Monitoring (methods)
• Freund's Adjuvant (administration & dosage)
• Humans
• Immunosuppression Therapy (methods)
• Interferon-gamma (blood)
• Lipids (administration & dosage)
• Mesenchymal Stem Cell Transplantation (methods)
• Mesenchymal Stem Cells (pathology)
• Rats
• Rats, Sprague-Dawley
• Treatment Outcome
• Tumor Necrosis Factor-alpha (blood)