Cardamonin induces autophagy and an antiproliferative effect through JNK activation in human colorectal carcinoma HCT116 cells.

Abstract

Cardamonin (2',4'-dihydroxy-6'-methoxychalcone) is derived from Alpinia katsumadai Hayata (Zingiberaceae), a plant that has been used in Traditional Chinese Medicine for thousands of years. Several anticancer agents have been reported to induce autophagy, which either protects cells or further sensitizes cells to drug treatment. However, the possible autophagic and antiproliferative effects of cardamonin on the human colorectal carcinoma HCT116 cell line are unclear. In the present study, experiments were conducted to determine the effects of cardamonin on cell proliferation, cell cycle distribution, and stimulation of autophagy in cultures of the HCT116 cell line. The results showed that cardamonin inhibited cell proliferation, induced G2/M phase cell cycle arrest, and enhanced autophagy in HCT116 cells. We found evidence that cardamonin-induced autophagic and antiproliferative effects are regulated by the tumor protein p53. We also found that the enhanced activation of c-Jun N-terminal kinase (JNK) by cardamonin was partially regulated by p53 and was critical for cardamonin-induced autophagic and antiproliferative effects in HCT116 cells. These findings suggest that cardamonin or other anticancer agents that increase p53/JNK-dependent stimulation of autophagy could be used to effectively treat patients with colorectal carcinoma.

• Authors: Young-Joo Kim, Ki Sung Kang, Kyung-Chul Choi, Hyeonseok Ko
• Journal: Bioorganic & medicinal chemistry letters (Bioorg Med Chem Lett) Vol. 25 Issue 12 Pg. 2559-64 (Jun 15 2015) ISSN: 1464-3405 [Electronic] England
• PMID: 25959811 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2015 Elsevier Ltd. All rights reserved.

Chemical References

• Antineoplastic Agents
• Chalcones
• RNA, Small Interfering
• Tumor Suppressor Protein p53
• Mitogen-Activated Protein Kinase 8
• cardamonin

Topics

• Antineoplastic Agents (chemistry, pharmacology)
• Apoptosis (drug effects)
• Cell Proliferation (drug effects)
• Chalcones (chemistry, pharmacology)
• Colorectal Neoplasms (metabolism, pathology)
• G2 Phase Cell Cycle Checkpoints (drug effects)
• HCT116 Cells
• Humans
• M Phase Cell Cycle Checkpoints (drug effects)
• Mitogen-Activated Protein Kinase 8 (metabolism)
• RNA Interference
• RNA, Small Interfering (metabolism)
• Tumor Suppressor Protein p53 (antagonists & inhibitors, genetics, metabolism)