Hydrogen sulfide and hypoxia-induced changes in TASK (K2P3/9) activity and intracellular Ca(2+) concentration in rat carotid body glomus cells.

Abstract

Acute hypoxia depolarizes carotid body chemoreceptor (glomus) cells and elevates intracellular Ca(2+) concentration ([Ca(2+)]i). Recent studies suggest that hydrogen sulfide (H2S) may serve as an oxygen sensor/signal in the carotid body during acute hypoxia. To further test such a role for H2S, we studied the effects of H2S on the activity of TASK channel and [Ca(2+)]i, which are considered important for mediating the glomus cell response to hypoxia. Like hypoxia, NaHS (a H2S donor) inhibited TASK activity and elevated [Ca(2+)]i. To inhibit the production of H2S, glomus cells were incubated (3h) with inhibitors of cystathionine-β-synthase and cystathionine-γ-lyase (DL-propargylglycine, aminooxyacetic acid, β-cyano-L-alanine; 0.3 mM). SF7 fluorescence was used to assess the level of H2S production. The inhibitors blocked L-cysteine- and hypoxia-induced elevation of SF7 fluorescence intensity. In cells treated with the inhibitors, hypoxia produced an inhibition of TASK activity and a rise in [Ca(2+)]i, similar in magnitude to those observed in control cells. L-cysteine produced no effect on TASK activity or [Ca(2+)]i and did not affect hypoxia-induced inhibition of TASK and elevation of [Ca(2+)]i. These findings suggest that under normal conditions, H2S is not a major signal in hypoxia-induced modulation of TASK channels and [Ca(2+)]i in isolated glomus cells.

• Authors: Donghee Kim, Insook Kim, Jiaju Wang, Carl White, John L Carroll
• Journal: Respiratory physiology & neurobiology (Respir Physiol Neurobiol) Vol. 215 Pg. 30-8 (Aug 15 2015) ISSN: 1878-1519 [Electronic] Netherlands
• PMID: 25956223 (Publication Type: Journal Article, Research Support, N.I.H., Extramural)
• Copyright: Copyright © 2015 Elsevier B.V. All rights reserved.

Chemical References

• 32-ascomycinyloxyacetic acid
• Alkynes
• Gasotransmitters
• Nerve Tissue Proteins
• Potassium Channels, Tandem Pore Domain
• Sodium Compounds
• potassium channel subfamily K member 3
• sodium hydride
• propargylglycine
• Calcium
• Glycine
• Tacrolimus
• Hydrogen Sulfide

Topics

• Alkynes (pharmacology)
• Analysis of Variance
• Animals
• Animals, Newborn
• Calcium (metabolism)
• Carotid Body (cytology)
• Chemoreceptor Cells (cytology, drug effects)
• Dose-Response Relationship, Drug
• Gasotransmitters (pharmacology)
• Glycine (analogs & derivatives, pharmacology)
• Hydrogen Sulfide (pharmacology)
• Hypoxia (metabolism)
• Intracellular Fluid (drug effects)
• Membrane Potentials (drug effects)
• Nerve Tissue Proteins
• Patch-Clamp Techniques
• Potassium Channels, Tandem Pore Domain (metabolism)
• Rats
• Rats, Sprague-Dawley
• Sodium Compounds
• Tacrolimus (analogs & derivatives, pharmacology)
• Time Factors