[Effects of Jisuikang on Nogo-NgR gene expression in spinal cord rats with injury].

Abstract

OBJECTIVE:

To study the effects of Jisuikang (Chinese characters) on Nogo-NgR gene expression, and to explore the protective effects and mechanism of Jisuikang (Chinese characters) on spinal cord injury in rats.

METHODS:

One hundred eighty female rats were randomly assigned to 6 groups(30 rats per group). Sham group: T10 lamina was resected only and spinal cord was untreated. Model group: spine cord injury (SCI) was created with a modified impinger of Allen's by impacting on the T10 spinal cord. Prednisolone group: Prednisolone (0.06 g/kg) was given by intragastric administration at a time interval of 24 hours after operation. The Jisuikang (Chinese characters) high, moderate and low dose groups: Jisuikang (Chinese characters) was supplied with different dose (50 g/kg, 25 g/kg, 12.5 g/kg) by intragastric administration in rats after operation,for the first time at 30 min after surgery. Animals were killed 3, 7, 14 days after surgery. The expression levels of Nogo-A and NgR were observed by Western Blot and Real-time PCR.

RESULTS:

The expression of Nogo-A and NgR was at the basic level at all time points in sham group. Compared with model group, the protein expression levels of Nogo-A and NgR in sham, prednisolone, Jisuikang (Chinese characters) moderate dose groups were statistically significant at all time points (P < 0.05). No difference was found in Jisuikang (Chinese characters) high and low dose groups (P > 0.05). Three days after surgery, the mRNA levels of Nogo-A and NgR in treatment group were significantly lower than that in model group (P < 0.01); 7 days after surgery,Nogo-A and NgR mRNA expression were dramatically upregulated and peaked; 14 days after operation, the expression was decreased, but still significantly higher than that in other treatment groups (P < 0.01). Prednisolone and Jisuikang (Chinese characters) moderate dose groups showed the most significant effects among all groups,but there was no statistically significant difference between two groups (P > 0.05).

CONCLUSION:

The decoction Jisuikang (Chinese characters) can promote the nerve cell regeneration by regulating Nogo-A and NgR gene expression, activating Nogo- NgR signaling pathways after acute spinal cord injury.

• Authors: Wu-Lin You, Ya-Feng Zhang, Jian-Feng Chen, Heng Yin, Jun-Feng Yang, Jian-Wei Wang
• Journal: Zhongguo gu shang = China journal of orthopaedics and traumatology (Zhongguo Gu Shang) Vol. 28 Issue 3 Pg. 235-9 (Mar 2015) ISSN: 1003-0034 [Print] China
• PMID: 25936193 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• GPI-Linked Proteins
• Myelin Proteins
• Nogo Proteins
• Nogo Receptor 1
• RTN4 protein, human
• RTN4R protein, human
• Receptors, Cell Surface
• Rtn4 protein, rat

Topics

• Animals
• Female
• GPI-Linked Proteins (analysis, genetics, physiology)
• Medicine, Chinese Traditional
• Myelin Proteins (analysis, genetics, physiology)
• Nerve Regeneration (drug effects)
• Nogo Proteins
• Nogo Receptor 1
• Rats
• Rats, Sprague-Dawley
• Receptors, Cell Surface (analysis, genetics, physiology)
• Signal Transduction (drug effects)
• Spinal Cord Injuries (drug therapy, metabolism)