Drospirenone induces decidualization in human eutopic endometrial stromal cells and reduces DNA synthesis of human endometriotic stromal cells.

Abstract

OBJECTIVE:

To investigate the in vitro effect of drospirenone on human eutopic endometrial (EuSC) and ectopic endometriotic stromal cells (EcSC).

DESIGN:

Comparative and laboratory study. The experimental procedures were approved by the Institutional Review Board of the University of Tokyo (registration no. 0324-4).

SETTING:

University research laboratory.

PATIENTS(S):

Eight patients undergoing hysterectomy for benign gynecologic disease and 19 patients undergoing cystectomy or adnectomy for endometriosis.

INTERVENTION(S):

EuSC and EcSC were treated with drospirenone.

MAIN OUTCOME MEASURE(S):

For the analysis of decidualization of EuSC, cells were observed using microscopy, and the production of PRL was measured using enzyme immunoassay. For the analysis of DNA synthesis of EcSC, 5-bromo-2'-deoxyuridine incorporation was measured by ELISA. Cells in apoptosis were detected and measured by flow cytometry.

RESULT(S):

Drospirenone induced decidualization in EuSC, and the induction was negated by RU486. Drospirenone reduced DNA synthesis on EcSC, and this reduction was negated by RU486 or P receptor silencing, but not by aldosterone or mineralocorticoid receptor silencing. Drospirenone did not cause EcSC to undergo apoptosis.

CONCLUSION(S):

Our study demonstrates the direct effects of drospirenone: decidualization of EuSC and reduced DNA synthesis of EcSC, but it does not cause EsSC apoptosis.

• Authors: Mariko Miyashita, Kaori Koga, Gentaro Izumi, Tomoko Makabe, Akiko Hasegawa, Yasushi Hirota, Tetsuya Hirata, Miyuki Harada, Tomoyuki Fujii, Yutaka Osuga
• Journal: Fertility and sterility (Fertil Steril) Vol. 104 Issue 1 Pg. 217-24.e2 (Jul 2015) ISSN: 1556-5653 [Electronic] United States
• PMID: 25935489 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2015 American Society for Reproductive Medicine. Published by Elsevier Inc. All rights reserved.

Chemical References

• Androstenes
• drospirenone

Topics

• Adult
• Androstenes (pharmacology)
• Apoptosis (drug effects, physiology)
• Cell Proliferation (drug effects, physiology)
• Cells, Cultured
• DNA Replication (drug effects, physiology)
• Decidua (drug effects, metabolism)
• Dose-Response Relationship, Drug
• Endometriosis (metabolism, pathology)
• Endometrium (cytology, drug effects, metabolism)
• Female
• Humans
• Middle Aged
• Stromal Cells (drug effects, metabolism)