Abstract | INTRODUCTION: METHODS: We quantified biofilm formation in the presence of ethanol and isopropyl alcohol in six different, well-characterized strains of Staphylococcus epidermidis and Staphylococcus aureus. After 24 h of biofilm development, each strain was exposed to normal saline (NS), ethanol, or isopropyl alcohol (40%, 60%, 80% and 95%) for additional 24 h incubation. Adherent biofilms were stained and optical density was determined. Viability of strains was also determined after alcohol exposure. RESULTS:
Ethanol increased biofilm formation in all six strains compared to normal saline (p < 0.05). There was increased biofilm formation with increasing ethanol concentration. Isopropyl alcohol also increased biofilm formation with increasing alcohol concentration in all six strains (p < 0.01 vs NS). The slime-negative, chemical mutant strain of S. epidermidis increased biofilm formation after exposure to both alcohols, likely reverting back its primary phenotype through modulation of the intercellular adhesin repressor. All strains demonstrated viability after exposure to each alcohol concentration, though viability was decreased. CONCLUSION:
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Authors | Megan K Luther, Sarah Bilida, Leonard A Mermel, Kerry L LaPlante |
Journal | Infectious diseases and therapy
(Infect Dis Ther)
Vol. 4
Issue 2
Pg. 219-26
(Jun 2015)
ISSN: 2193-8229 [Print] New Zealand |
PMID | 25935134
(Publication Type: Journal Article)
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