Abstract |
Vascular endothelial dysfunction and inflammatory response are early events during initiation and progression of atherosclerosis. In vitro studies have described that CIT markedly upregulates expressions of ICAM-1 and VCAM-1 of endothelial cells, which result from NF-κB activation induced by CIT. In order to determine whether it plays a role in atherogenesis in vivo, we conducted the study to investigate the effects of CIT on atherosclerotic plaque development and inflammatory response in apolipoprotein E deficient ( apoE-/-) mice. Five-week-old apoE-/- mice were fed high-fat diets and treated with CIT for 15 weeks, followed by assay of atherosclerotic lesions. Nitric oxide (NO), vascular endothelial growth factor ( VEGF) and endothelin-1 (ET-1) were detected in serum. Levels of intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), VEGF, and ET-1 in plaque areas of artery walls were examined. NF-κB p65 expression and NF-κB activation in aorta also were assessed. CIT treatment significantly augmented atherosclerotic plaques and increased expressions of ICAM-1, VCAM-1, VEGF and ET-1 in aorta. Mechanistic studies showed that activation of NF-κB was significantly elevated by CIT treatment, indicating the effect of CIT on atherosclerosis may be regulated by activation of NF-κB.
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Authors | Hai-Feng Hou, Na Yuan, Qing Guo, Tao Sun, Cheng Li, Jian-Bao Liu, Qun-Wei Li, Bao-Fa Jiang |
Journal | PloS one
(PLoS One)
Vol. 10
Issue 5
Pg. e0125956
( 2015)
ISSN: 1932-6203 [Electronic] United States |
PMID | 25933220
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Apolipoproteins E
- Aurovertins
- Endothelin-1
- Lipids
- NF-kappa B
- Vascular Cell Adhesion Molecule-1
- Vascular Endothelial Growth Factor A
- Intercellular Adhesion Molecule-1
- Nitric Oxide
- citreoviridin
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Topics |
- Animals
- Aorta
(drug effects, pathology)
- Apolipoproteins E
(deficiency)
- Atherosclerosis
(blood, complications)
- Aurovertins
(toxicity)
- Body Weight
(drug effects)
- Diet, High-Fat
- Endothelin-1
(metabolism)
- Endothelium, Vascular
(drug effects, pathology, physiopathology)
- Hypercholesterolemia
(blood, complications)
- Inflammation
(complications)
- Intercellular Adhesion Molecule-1
(metabolism)
- Lipids
(blood)
- Male
- Mice, Inbred C57BL
- NF-kappa B
(metabolism)
- Nitric Oxide
(metabolism)
- Up-Regulation
(drug effects)
- Vascular Cell Adhesion Molecule-1
(metabolism)
- Vascular Endothelial Growth Factor A
(metabolism)
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