Targeted chemotherapy of visceral leishmaniasis by lactoferrin-appended amphotericin B-loaded nanoreservoir: in vitro and in vivo studies.

Abstract

AIM:

Exploitation of lactoferrin-appended amphotericin B bearing nanoreservoir (LcfPGNP-AmB) for targeted eradication of Leishmania donovani.

MATERIALS & METHODS:

LcfPGNP-AmB was architechtured through ionic adsorption of lactoferrin over core poly (d,l-lactide-co-glycolide) nanoparticles and characterized. Anti-Leishmania activity in visceral leishmaniasis models, immunomodulatory potential, biodistribution and toxicity profile were also assessed.

RESULTS:

LcfPGNP-AmB (size, 196.0 ± 5.28 nm; zeta-potential, +21.7 ± 1.52 mV; encapsulation efficiency, ∼89%) showed reduced toxicity, increased protective proinflammatory mediators expression and down-regulation of disease-promoting cytokines. Biodistribution study illustrated preferential accumulation of LcfPGNP-AmB in liver and spleen. LcfPGNP-AmB showed augmented antileishmanial activity by significantly reducing (∼88%) splenic parasite burden of infected hamsters, compared with commercial-formulations.

CONCLUSION:

Superior efficacy, desired stability and reliable safety of cost-effective LcfPGNP-AmB, suggest its potential for leishmaniasis therapeutics.

• Authors: Shalini Asthana, Pramod K Gupta, Anil K Jaiswal, Anuradha Dube, Manish K Chourasia
• Journal: Nanomedicine (London, England) (Nanomedicine (Lond)) Vol. 10 Issue 7 Pg. 1093-109 ( 2015) ISSN: 1748-6963 [Electronic] England
• PMID: 25929567 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Drug Carriers
• Trypanocidal Agents
• Amphotericin B
• Lactoferrin

Topics

• Amphotericin B (administration & dosage, pharmacokinetics, pharmacology, therapeutic use)
• Animals
• Cell Line
• Cricetinae
• Drug Carriers (chemistry, metabolism)
• Drug Delivery Systems
• Lactoferrin (chemistry, metabolism)
• Leishmania donovani (drug effects)
• Leishmaniasis, Visceral (drug therapy, parasitology)
• Macrophages (parasitology)
• Male
• Mice
• Nanoparticles (chemistry, metabolism, ultrastructure)
• Rats, Wistar
• Spleen (parasitology)
• Tissue Distribution
• Trypanocidal Agents (administration & dosage, pharmacokinetics, pharmacology, therapeutic use)