Abstract | OBJECTIVE: METHODS: Fifty Wistar rats were randomly assigned to the control group, MODS model group and DCQD treated group. The rats in MODS model group and DCQD treated group were injected Escherichia coli (E. coli) suspension into abdominal cavity under sterile condition. The DCQD treated group was gavaged with DCQD 2 days before the E. coli suspension was injected. Twenty-four hours after injection, the proximal segment of intestine was resected and studied by immunohistofluorescence using vesicular acetylcholine transporter, vasoactive intestinal polypeptide (VIP), substance P (SP) and neuronal nitric oxide synthase (nNOS) antibodies. The whole-mount preparations were observed by laser scanning confocal microscope to detect the changes of quantity and fluorescence integral optical density (IOD) value of intestine enteric nerves. RESULTS: Compared with the control group, the quantity and IOD value of acetylcholine (ACh), VIP, SP and nitric oxide (NO) nerves of intestine in the MODS group were significantly decreased (P<0.01), and the network of enteric nerves was remarkably disrupted. Compared with the MODS group, the quantity and fluorescence IOD value of ACh, VIP, SP and NO nerves in the DCQD group were significantly increased (P<0.01), and the network of enteric nerves was remarkably recovered. CONCLUSION: DCQD can protect and repair damage in the network of ACh, SP, NO and VIP nerves in rats with MODS, which may be one of mechanisms involved in promoting gastrointestinal motility by DCQD.
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Authors | Ming-zheng Xie, Qing-hui Qi, Sheng-lin Zhang, Ming-ming Wei |
Journal | Chinese journal of integrative medicine
(Chin J Integr Med)
Vol. 21
Issue 8
Pg. 624-9
(Aug 2015)
ISSN: 1672-0415 [Print] China |
PMID | 25920537
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Plant Extracts
- dachengqi decoction
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Topics |
- Animals
- Enteric Nervous System
(drug effects, pathology)
- Female
- Male
- Multiple Organ Failure
(pathology)
- Plant Extracts
(pharmacology)
- Rats
- Rats, Wistar
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