WEHI-3 cells inhibit adipocyte differentiation in 3T3-L1 cells.

Abstract

By investigating the anti-adipogenic effects of WEHI-3 cells - a murine acute myelomonocytic leukemia cell line - we sought to improve the efficiency of hematopoietic stem cell transplantation (HSCT). Analysis of Oil Red O staining and the expression of adipogenic genes, including PPARγ, C/EBPα, FAS and LPL, indicated that WEHI-3 cells significantly inhibited 3T3-L1 mouse preadipocyte cells from differentiating into adipocytes. In vivo, fat vacuoles in mice injected with WEHI-3 cells were also remarkably reduced in the murine bone marrow pimelosis model. Moreover, the key gene in the Rho signaling pathway, ROCKII, and the key gene in the Wnt signaling pathway, β-catenin, were both upregulated compared with the control group. siRNA-mediated knockdown of ROCKII and β-catenin reversed these WEHI-3-mediated anti-adipogenic effects. Taken together, these data suggest that WEHI-3 cells exert anti-adipogenic effects and that both ROCKII and β-catenin are involved in this process.

• Authors: Jing Lai, Gexiu Liu, Guoyao Yan, Dongmei He, Ying Zhou, Shengting Chen
• Journal: Biochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 462 Issue 2 Pg. 105-11 (Jun 26 2015) ISSN: 1090-2104 [Electronic] United States
• PMID: 25911323 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2015 Elsevier Inc. All rights reserved.

Chemical References

• CCAAT-Enhancer-Binding Protein-alpha
• CTNNB1 protein, mouse
• PPAR gamma
• RNA, Small Interfering
• beta Catenin
• Rock2 protein, mouse
• rho-Associated Kinases

Topics

• 3T3-L1 Cells
• Adipocytes (cytology, metabolism)
• Adipogenesis (genetics, physiology)
• Animals
• Bone Marrow Cells (cytology, metabolism)
• CCAAT-Enhancer-Binding Protein-alpha (metabolism)
• Cell Differentiation (genetics, physiology)
• Cell Line
• Coculture Techniques
• Female
• Gene Expression Regulation, Developmental
• Gene Knockdown Techniques
• Hematopoietic Stem Cell Transplantation
• Mice
• Mice, Inbred BALB C
• Models, Biological
• PPAR gamma (metabolism)
• RNA, Small Interfering (genetics)
• Signal Transduction
• Wnt Signaling Pathway
• beta Catenin (antagonists & inhibitors, genetics, metabolism)
• rho-Associated Kinases (antagonists & inhibitors, genetics, metabolism)