Ginsenoside Ro suppresses interleukin-1β-induced apoptosis and inflammation in rat chondrocytes by inhibiting NF-κB.

Abstract

This study investigated effects of Ginsenoside Ro (Ro) on interleukin-1β (IL-1β)-induced apoptosis and inflammation in rat chondrocytes. The rat chondrocytes were co-treated with IL-1β (10 ng·kg(-1)) and Ro (50, 100 and 200 μmol·L(-1)) for 48 h. Chondrocytes viability was detected by the MTT assay and Annexin V-FITC/PI dual staining assay. Caspase 3 activity was measured by using caspase 3 colorimetric assay kit. Apoptosis related proteins Bax, Bad, Bcl-xL, PCNA, p53 and phospho-p53, along with inflammation related protein MMP 3, MMP 9 and COX-2, and the expression of phospho-NF-κB p65 were assayed by western blotting analyses. Ro could improve IL-1β-induced chondrocytes viability. Ro could suppress IL-1β-induced apoptosis by inhibiting levels of Bax and Bad, decreasing p53 phosphorylation and promoting the expression of Bcl-xL and PCNA. Ro inhibited caspase 3 activity. IL-1β-induced inflammation and matrix degration were also alleviated by Ro with down-regulating the expression of MMP 3, MMP 9 and COX-2. Moreover, Ro inhibited NF-κB p65 phosphorylation induced by IL-1β. In conclusion, these results suggested Ro exerted anti-apoptosis and anti-inflammation in IL-1β-induced rat chondrocytes, which might be related to NF-κB signal pathway. Therefore, we propose that Ro might be a potential novel drug for the treatment of osteoarthritis.

• Authors: Xiao-Hong Zhang, Xian-Xiang Xu, Tao Xu
• Journal: Chinese journal of natural medicines (Chin J Nat Med) Vol. 13 Issue 4 Pg. 283-9 (Apr 2015) ISSN: 1875-5364 [Electronic] China
• PMID: 25908625 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2015 China Pharmaceutical University. Published by Elsevier B.V. All rights reserved.

Chemical References

• Anti-Inflammatory Agents
• Apoptosis Regulatory Proteins
• Ginsenosides
• Interleukin-1beta
• NF-kappa B
• ginsenoside Ro
• Cyclooxygenase 2
• Ptgs2 protein, rat
• Caspase 3
• Matrix Metalloproteinase 3
• Matrix Metalloproteinase 9

Topics

• Animals
• Anti-Inflammatory Agents (pharmacology)
• Apoptosis (drug effects)
• Apoptosis Regulatory Proteins (drug effects, metabolism)
• Caspase 3 (metabolism)
• Cell Survival (drug effects)
• Chondrocytes (cytology, drug effects)
• Cyclooxygenase 2 (drug effects, metabolism)
• Down-Regulation (drug effects)
• Drug Evaluation, Preclinical
• Ginsenosides (pharmacology)
• Inflammation (chemically induced, drug therapy)
• Interleukin-1beta (antagonists & inhibitors, pharmacology)
• Matrix Metalloproteinase 3 (drug effects, metabolism)
• Matrix Metalloproteinase 9 (drug effects)
• NF-kappa B (antagonists & inhibitors, drug effects, metabolism)
• Phosphorylation (drug effects)
• Rats
• Rats, Sprague-Dawley
• Signal Transduction (drug effects)