Prophylactic effects of asiaticoside-based standardized extract of Centella asiatica (L.) Urban leaves on experimental migraine: Involvement of 5HT1A/1B receptors.

Abstract

The present study aimed at evaluation of prophylactic efficacy and possible mechanisms of asiaticoside (AS) based standardized extract of Centella asiatica (L.) Urban leaves (INDCA) in animal models of migraine. The effects of oral and intranasal (i.n.) pretreatment of INDCA (acute and 7-days subacute) were evaluated against nitroglycerine (NTG, 10 mg·kg(-1), i.p.) and bradykinin (BK, 10 μg, intra-arterial) induced hyperalgesia in rats. Tail flick latencies (from 0 to 240 min) post-NTG treatment and the number of vocalizations post-BK treatment were recorded as a measure of hyperalgesia. Separate groups of rats for negative (Normal) and positive (sumatriptan, 42 mg·kg(-1), s.c.) controls were included. The interaction of INDCA with selective 5-HT1A, 5-HT1B, and 5-HT1D receptor antagonists (NAN-190, Isamoltane hemifumarate, and BRL-15572 respectively) against NTG-induced hyperalgesia was also evaluated. Acute and sub-acute pre-treatment of INDCA [10 and 30 mg·kg(-1) (oral) and 100 μg/rat (i.n.) showed significant anti-nociception activity, and reversal of the NTG-induced hyperalgesia and brain 5-HT concentration decline. Oral pre-treatment with INDCA (30 mg·kg(-1), 7 d) showed significant reduction in the number of vocalization. The anti-nociceptive effects of INDCA were blocked by 5-HT1A and 5-HT1B but not 5-HT1D receptor antagonists. In conclusion, INDCA demonstrated promising anti-nociceptive effects in animal models of migraine, probably through 5-HT1A/1B medicated action.

• Authors: Vijeta Bobade, Subhash L Bodhankar, Urmila Aswar, Mohan Vishwaraman, Prasad Thakurdesai
• Journal: Chinese journal of natural medicines (Chin J Nat Med) Vol. 13 Issue 4 Pg. 274-82 (Apr 2015) ISSN: 1875-5364 [Electronic] China
• PMID: 25908624 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2015 China Pharmaceutical University. Published by Elsevier B.V. All rights reserved.

Chemical References

• Centella asiatica extract
• Plant Extracts
• Receptors, Serotonin, 5-HT1
• Serotonin 5-HT1 Receptor Antagonists
• Triterpenes
• Nitroglycerin
• asiaticoside
• Bradykinin

Topics

• Administration, Intranasal
• Administration, Oral
• Animals
• Bradykinin
• Centella
• Female
• Hyperalgesia (chemically induced, prevention & control)
• Male
• Migraine Disorders (chemically induced, prevention & control)
• Models, Animal
• Nitroglycerin
• Nociception (drug effects)
• Plant Extracts
• Plant Leaves (chemistry)
• Pre-Exposure Prophylaxis
• Rats
• Rats, Wistar
• Reaction Time
• Receptors, Serotonin, 5-HT1 (drug effects)
• Serotonin 5-HT1 Receptor Antagonists (metabolism)
• Tail (physiology)
• Triterpenes (administration & dosage, pharmacology)