Abstract |
A simple, accurate and precise procedure for the quantitation of itazigrel (a potent lipophilic inhibitor of collagen and arachidonic acid-induced aggregation being studied for its effects on peripheral vascular disease) from granulated rodent diet is presented. The drug was extracted from rodent diet using methanol + water (80:20) following dissolution of the diet in water. Samples of the supernatant were injected into the HPLC and the eluent was monitored with a fluorescent detector (lambda ex = 320 and lambda em = 430) to achieve analytical specificity. Interday coefficients of variation of the calibration curve slope were +/- 6% on standards between 0 and 1000 micrograms/g. Potency and homogeneity of the drug spiked diet prepared over a 1 year interval at 70, 200 and 600 micrograms/g was 99.3 +/- 2.5%, 100 +/- 1.8%, and 101 +/- 1.9% of label, respectively. Samples prepared for chromatography were stable for 24 h at 20 degrees C, and drug in diet was stable for 102 days when protected from light and stored at 20 degrees C.
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Authors | P A Bombardt, B E Bothwell, S K Closson, W J Adams |
Journal | Biomedical chromatography : BMC
(Biomed Chromatogr)
Vol. 3
Issue 4
Pg. 180-2
(Jul 1989)
ISSN: 0269-3879 [Print] England |
PMID | 2590729
(Publication Type: Journal Article)
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Chemical References |
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Topics |
- Animal Feed
(analysis)
- Animals
- Chromatography, High Pressure Liquid
- Rodentia
- Spectrometry, Fluorescence
- Temperature
- Thiazoles
(analysis, toxicity)
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