Postoperative prognosis prediction of pancreatic cancer with seven microRNAs.

Abstract

OBJECTIVES:

The aim of this study was to investigate microRNAs related to postoperative prognosis of pancreatic cancer by applying microarray analysis to stored surgical specimens.

METHODS:

We retrospectively analyzed the relationship between microRNA expression and postoperative survival of pancreatic cancer patients in Samsung Medical Center. After the correlation of microRNA expression between paraffin-embedded and fresh-frozen specimens was confirmed, we applied NanoString nCounter system to formalin fixed paraffin-embedded surgical specimens between 1995 September and 2010 June.

RESULTS:

Paired fresh and paraffin-embedded samples from 9 patients showed a high correlation (correlation coefficient > 0.91). Among 734 microRNAs, 7 microRNAs were found to be related to postoperative overall survival of 221 pancreatic cancer patients: hsa-miR-99a, hsa-miR-150, hsa-miR-194, hsa-miR-375, hsa-miR-664, hsa-miR-342-3p, and hsa-miR-487b (P < 0.01). The prognostic microRNA signature was consistent after adjustment of clinical parameters: resection status, stages, age, sex, adjuvant treatment, pathological differentiation and postoperative carbohydrate antigen 19-9 levels.

CONCLUSIONS:

We found a prediction model for postoperative prognosis of pancreatic cancer using 7 microRNA expression patterns from archived formalin-fixed paraffin-embedded surgical specimens. The methods for microRNA detection and new microRNAs detected in this study provide new insights for new research of surgically resected pancreatic cancer samples and microRNAs.

• Authors: Kwang Hyuck Lee, Jong Kyun Lee, Dong Wook Choi, In-Gu Do, Insuk Sohn, Kee-Taek Jang, Sin-Ho Jung, Jin Seok Heo, Seong Ho Choi, Kyu Taek Lee
• Journal: Pancreas (Pancreas) Vol. 44 Issue 5 Pg. 764-8 (Jul 2015) ISSN: 1536-4828 [Electronic] United States
• PMID: 25906450 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Biomarkers, Tumor
• MicroRNAs

Topics

• Aged
• Biomarkers, Tumor (genetics)
• Disease Progression
• Disease-Free Survival
• Female
• Frozen Sections
• Gene Expression Profiling (methods)
• Humans
• Male
• MicroRNAs (genetics)
• Middle Aged
• Neoplasm Recurrence, Local
• Oligonucleotide Array Sequence Analysis
• Pancreatectomy (adverse effects, mortality)
• Pancreatic Neoplasms (genetics, mortality, pathology, surgery)
• Paraffin Embedding
• Predictive Value of Tests
• Proportional Hazards Models
• Retrospective Studies
• Risk Factors
• Treatment Outcome