Abstract | OBJECTIVE:
Dibutyl phthalate (DBP) is one of the most widely used phthalate esters, and it is ubiquitous in the environment. DBP and its major metabolite, monobutyl phthalate (MBP), change steroid biosynthesis and impair male reproductive function. However, the regulatory mechanism underlying the steroid biosynthesis disruption by MBP is still unclear. METHODS: RESULTS: Our results reveal that MBP inhibited progesterone production. At the same time, StAR mRNA and protein were decreased after MBP exposure. Furthermore, electrophoretic mobility shift assay showed that DNA-binding affinity of transcription factors (SF-1 and GATA-4) was decreased in a dose-dependent manner after MBP treatments. Western blot tests next confirmed that protein of SF-1 was decreased, but GATA-4 protein was unchanged. However, phosphorylated GATA-4 protein was decreased with 800 μM of MBP. CONCLUSIONS: This study reveals an important and novel mechanism whereby SF-1 and GATA-4 may regulate StAR during MBP-induced steroidogenesis disruption.
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Authors | Y Hu, C Dong, M Chen, Y Chen, A Gu, Y Xia, H Sun, Z Li, Y Wang |
Journal | Journal of endocrinological investigation
(J Endocrinol Invest)
Vol. 38
Issue 8
Pg. 875-84
(Aug 2015)
ISSN: 1720-8386 [Electronic] Italy |
PMID | 25903692
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- GATA4 Transcription Factor
- Gata4 protein, mouse
- Phosphoproteins
- Phthalic Acids
- Steroidogenic Factor 1
- Transcription Factors
- steroidogenic acute regulatory protein
- monobutyl phthalate
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Topics |
- Animals
- Cell Survival
(drug effects, physiology)
- Dose-Response Relationship, Drug
- GATA4 Transcription Factor
(metabolism)
- Leydig Cell Tumor
(metabolism)
- Mice
- Phosphoproteins
(metabolism)
- Phthalic Acids
(pharmacology)
- Steroidogenic Factor 1
(metabolism)
- Transcription Factors
(physiology)
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