The antitumor activity of PNA modified vinblastine cationic liposomes on Lewis lung tumor cells: In vitro and in vivo evaluation.

Abstract

Non-small cell lung cancer (NSCLC) is one of the frequently-occurring disease in the world, and the treatment effects are usually unsatisfactory. Vinblastine is an anti-microtubule drug in clinic. In this study, a nanostructured liposome was designed and prepared for treating NSCLC. In the liposomes, peanut agglutinin (PNA) was modified on the liposomal surface, 3-(N-(N',N'-dimethylaminoethane)carbamoyl) cholesterol was used as cationic materials, and vinblastine was encapsulated in the aqueous core of liposomes, respectively. The PNA modified vinblastine cationic liposomes were approximately 100 nm in size with a positive potential. In vitro results showed that the targeting liposomes could significantly enhance cellular uptake, selectively accumulate in LLT cells, and dramatically initiate apoptosis via activating pro-apoptotic proteins and apoptotic enzymes, thus leading to the strongest antitumor efficacy to LLT cells. In vivo results demonstrated that the targeting liposomes could display a prolonged circulation time in the blood, accumulate more drug in tumor location, and induce most of tumor cells apoptosis. As a result, a robust overall antitumor efficacy in tumor-bearing mice was observed subsequently. In conclusion, the chemotherapy using the PNA modified vinblastine cationic liposomes could provide a potential strategy for treating non-small cell lung cancer.

• Authors: Xue-Tao Li, Mei-Li He, Zhi-Yan Zhou, Ying Jiang, Lan Cheng
• Journal: International journal of pharmaceutics (Int J Pharm) Vol. 487 Issue 1-2 Pg. 223-33 (Jun 20 2015) ISSN: 1873-3476 [Electronic] Netherlands
• PMID: 25895716 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2015 Elsevier B.V. All rights reserved.

Chemical References

• Antineoplastic Agents, Phytogenic
• Apoptosis Regulatory Proteins
• Coumarins
• Liposomes
• Peanut Agglutinin
• Vinblastine

Topics

• Animals
• Antineoplastic Agents, Phytogenic (administration & dosage, pharmacology)
• Apoptosis Regulatory Proteins (metabolism)
• Carcinoma, Lewis Lung (drug therapy)
• Carcinoma, Non-Small-Cell Lung (drug therapy)
• Cell Line, Tumor
• Coumarins (chemistry)
• Drug Compounding
• Liposomes
• Mice
• Mice, Inbred C57BL
• Nanoparticles
• Particle Size
• Peanut Agglutinin (pharmacology)
• Vinblastine (administration & dosage, pharmacology)
• Xenograft Model Antitumor Assays