Quantitative analysis of PMLA nanoconjugate components after backbone cleavage.

Abstract

Multifunctional polymer nanoconjugates containing multiple components show great promise in cancer therapy, but in most cases complete analysis of each component is difficult. Polymalic acid (PMLA) based nanoconjugates have demonstrated successful brain and breast cancer treatment. They consist of multiple components including targeting antibodies, Morpholino antisense oligonucleotides (AONs), and endosome escape moieties. The component analysis of PMLA nanoconjugates is extremely difficult using conventional spectrometry and HPLC method. Taking advantage of the nature of polyester of PMLA, which can be cleaved by ammonium hydroxide, we describe a method to analyze the content of antibody and AON within nanoconjugates simultaneously using SEC-HPLC by selectively cleaving the PMLA backbone. The selected cleavage conditions only degrade PMLA without affecting the integrity and biological activity of the antibody. Although the amount of antibody could also be determined using the bicinchoninic acid (BCA) method, our selective cleavage method gives more reliable results and is more powerful. Our approach provides a new direction for the component analysis of polymer nanoconjugates and nanoparticles.

• Authors: Hui Ding, Rameshwar Patil, Jose Portilla-Arias, Keith L Black, Julia Y Ljubimova, Eggehard Holler
• Journal: International journal of molecular sciences (Int J Mol Sci) Vol. 16 Issue 4 Pg. 8607-20 (Apr 16 2015) ISSN: 1422-0067 [Electronic] Switzerland
• PMID: 25894227 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)

Chemical References

• Antineoplastic Agents
• Malates
• Nanoconjugates
• Polymers
• poly(malic acid)
• Trastuzumab

Topics

• Antineoplastic Agents (chemistry)
• Drug Delivery Systems
• Kinetics
• Malates (chemistry)
• Nanoconjugates (chemistry)
• Polymers (chemistry)
• Trastuzumab (chemistry)