The flavonoid morin from Moraceae induces apoptosis by modulation of Bcl-2 family members and Fas receptor in HCT 116 cells.
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| Abstract |
It is evident based on literature that flavonoids from fruit can safely modulate cancer cell biology and induce apoptosis. Therefore, we investigated the anticancer activity of morin, a flavonoid which is plentiful in twigs of mulberry focusing on apoptosis, and its mechanisms. Morin upregulated the Fas receptor, and activates caspase-8, -9 and -3 in HCT-116 cells. Morin also activates Bid, and induced the loss of mitochondrial membrane potential (MMP, ∆Ψm) with Bax protein activation and cytochrome c release. In addition, morin induced ROS generation which was not blocked by N-acetylcysteine. Morin also suppressed Bcl-2 and cIAP-1, anti-apoptotic proteins, which may contribute to augmentation of morin-triggered apoptosis. As an upstream signaling pathway, suppressed Akt activity by morin was associated to apoptosis. This study suggests that morin induces caspase-dependent apoptosis through extrinsic pathway by upregulating Fas receptor as well as through the intrinsic pathway by modulating Bcl-2 and IAP family members, and ROS generation, and that Akt is the critical upstream signaling that regulates the apoptotic effect of morin in human colon cancer HCT-116 cells.
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| Authors | Hwang-Bo Hyun, Won Sup Lee, Se-Il Go, Arulkumar Nagappan, Cheol Park, Min Ho Han, Su Hyun Hong, Gonsup Kim, Gi Young Kim, Jaehun Cheong, Chung Ho Ryu, Sung Chul Shin, Yung Hyun Choi |
| Journal | International journal of oncology (Int J Oncol) Vol. 46 Issue 6 Pg. 2670-8 ( 2015) ISSN: 1791-2423 [Electronic] Greece |
| PMID | 25892545 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't) |
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