Identification of ABC transporter genes conferring combined pleuromutilin-lincosamide-streptogramin A resistance in bovine methicillin-resistant Staphylococcus aureus and coagulase-negative staphylococci.

Abstract

The aim of this study was to investigate the genetic basis of combined pleuromutilin-lincosamide-streptogramin A resistance in 26 unrelated methicillin-resistant Staphylococcus aureus (MRSA) and coagulase-negative staphylococci (CoNS) from dairy cows suffering from mastitis. The 26 pleuromutilin-resistant staphylococcal isolates were screened for the presence of the genes vga(A), vga(B), vga(C), vga(E), vga(E) variant, sal(A), vmlR, cfr, lsa(A), lsa(B), lsa(C), and lsa(E) by PCR. None of the 26 isolates carried the genes vga(B), vga(C), vga(E), vga(E) variant, vmlR, cfr, lsa(A), lsa(B), or lsa(C). Two Staphylococcus haemolyticus and single Staphylococcus xylosus, Staphylococcus lentus, and Staphylococcus hominis were vga(A)-positive. Twelve S. aureus, two Staphylococcus warneri, as well as single S. lentus and S. xylosus carried the lsa(E) gene. Moreover, single S. aureus, S. haemolyticus, S. xylosus, and Staphylococcus epidermidis were positive for both genes, vga(A) and lsa(E). The sal(A) gene was found in a single Staphylococcus sciuri. All ABC transporter genes were located in the chromosomal DNA, except for a plasmid-borne vga(A) gene in the S. epidermidis isolate. The genetic environment of the lsa(E)-positive isolates was analyzed using previously described PCR assays. Except for the S. warneri and S. xylosus, all lsa(E)-positive isolates harbored a part of the previously described enterococcal multiresistance gene cluster. This is the first report of the novel lsa(E) gene in the aforementioned bovine CoNS species. This is also the first identification of the sal(A) gene in a S. sciuri from a case of bovine mastitis. Moreover, the sal(A) gene was shown to also confer pleuromutilin resistance.

• Authors: Sarah Wendlandt, Kristina Kadlec, Andrea T Feßler, Stefan Schwarz
• Journal: Veterinary microbiology (Vet Microbiol) Vol. 177 Issue 3-4 Pg. 353-8 (Jun 12 2015) ISSN: 1873-2542 [Electronic] Netherlands
• PMID: 25891423 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2015 Elsevier B.V. All rights reserved.

Chemical References

• ATP-Binding Cassette Transporters
• Anti-Bacterial Agents
• Coagulase
• Diterpenes
• Lincosamides
• Polycyclic Compounds
• Streptogramins
• pleuromutilin
• Streptogramin A
• Methicillin

Topics

• ATP-Binding Cassette Transporters (genetics)
• Animals
• Anti-Bacterial Agents (pharmacology)
• Cattle
• Coagulase (genetics)
• Diterpenes (pharmacology)
• Drug Resistance, Multiple, Bacterial (genetics)
• Female
• Lincosamides (pharmacology)
• Mastitis, Bovine (drug therapy, microbiology)
• Methicillin (pharmacology)
• Methicillin-Resistant Staphylococcus aureus (drug effects, genetics)
• Microbial Sensitivity Tests
• Polycyclic Compounds
• Staphylococcal Infections (microbiology, veterinary)
• Staphylococcus (drug effects, enzymology)
• Staphylococcus epidermidis (drug effects, genetics)
• Staphylococcus haemolyticus (drug effects, genetics)
• Staphylococcus hominis (drug effects, genetics)
• Streptogramin A (pharmacology)
• Streptogramins (pharmacology)