Abstract |
The survival of cells in the 3D scaffold until the ingrowth of blood vessels is one of the most important challenges in tissue engineering for producing a clinically relevant volume of tissue. In this study, perfluorooctane emulsion ( oxygen carrier)-loaded hollow microparticles (PFO-HPs) were prepared as a scaffolding system which can allow timely release of oxygen to cells adhered on the HPs to prevent cell necrosis in a hypoxic environment (inherently created in tissue engineered 3D constructs) until new blood vessels are formed in the 3D cell construct, and thus may produce appropriate tissues/organs with a clinically relevant volume. In the in vitro cell culture and the in vivo animal study, it was observed that the cells initially seeded on the PFO-HPs remained alive for approximately 10 days in a hypoxic environment (in vitro), and the cells were also found throughout the implanted whole matrix without a necrotic center until the infiltration of blood vessels (at 14 days after implantation; in vivo), probably due to the sufficient release of oxygen from the PFO-HPs for an adequate time period. Based on these results, the cell-based PFO-HPs can be a promising system to produce a clinically applicable large tissue mass.
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Authors | Hye-Young Lee, Hae-Won Kim, Jin Ho Lee, Se Heang Oh |
Journal | Biomaterials
(Biomaterials)
Vol. 53
Pg. 583-91
( 2015)
ISSN: 1878-5905 [Electronic] Netherlands |
PMID | 25890754
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2015 Elsevier Ltd. All rights reserved. |
Chemical References |
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Topics |
- Animals
- Cell Hypoxia
- Cell Survival
- Mice
- Mice, Inbred BALB C
- Microspheres
- Oxygen
(chemistry)
- Tissue Engineering
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