Xanthohumol-Mediated Suppression of Notch1 Signaling Is Associated with Antitumor Activity in Human Pancreatic Cancer Cells.

Abstract

Pancreatic cancer remains a lethal disease with limited treatment options. At the time of diagnosis, approximately 80% of these patients present with unresectable tumors caused by either locally advanced lesions or progressive metastatic growth. Therefore, development of novel treatment strategies and new therapeutics is needed. Xanthohumol (XN) has emerged as a potential compound that inhibits various types of cancer, but the molecular mechanism underlying the effects of XN remains unclear. In the present study, we have assessed the efficacy of XN on pancreatic cancer cell lines (AsPC-1, PANC-1, L3.6pl, MiaPaCa-2, 512, and 651) against cell growth in real time and using colony-forming assays. Treatment with XN resulted in reduction in cellular proliferation in a dose- and time-dependent manner. The growth suppression effect of XN in pancreatic cancer cell lines is due to increased apoptosis via the inhibition of the Notch1 signaling pathway, as evidenced by reduction in Notch1, HES-1, and survivin both at mRNA as well as protein levels. Notch1 promoter reporter analysis after XN treatment indicated that XN downregulates Notch promoter activity. Importantly, overexpression of active Notch1 in XN-treated pancreatic cancer cells resulted in negation of growth suppression. Taken together, these findings demonstrate, for the first time, that the growth suppressive effect of XN in pancreatic cancer cells is mainly mediated by Notch1 reduction.

• Authors: Selvi Kunnimalaiyaan, Jose Trevino, Susan Tsai, T Clark Gamblin, Muthusamy Kunnimalaiyaan
• Journal: Molecular cancer therapeutics (Mol Cancer Ther) Vol. 14 Issue 6 Pg. 1395-403 (Jun 2015) ISSN: 1538-8514 [Electronic] United States
• PMID: 25887885 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
• Copyright: ©2015 American Association for Cancer Research.

Chemical References

• Antineoplastic Agents
• BIRC5 protein, human
• Basic Helix-Loop-Helix Transcription Factors
• Flavonoids
• Homeodomain Proteins
• Inhibitor of Apoptosis Proteins
• NOTCH1 protein, human
• Propiophenones
• Receptor, Notch1
• Survivin
• Transcription Factor HES-1
• HES1 protein, human
• xanthohumol

Topics

• Antineoplastic Agents (pharmacology)
• Apoptosis (drug effects)
• Basic Helix-Loop-Helix Transcription Factors (genetics, metabolism)
• Blotting, Western
• Cell Line
• Cell Line, Tumor
• Cell Proliferation (drug effects)
• Cell Survival (drug effects)
• Dose-Response Relationship, Drug
• Flavonoids (pharmacology)
• Gene Expression Regulation, Neoplastic (drug effects)
• Homeodomain Proteins (genetics, metabolism)
• Humans
• Inhibitor of Apoptosis Proteins (genetics, metabolism)
• Pancreatic Neoplasms (genetics, metabolism, pathology)
• Propiophenones (pharmacology)
• Receptor, Notch1 (genetics, metabolism)
• Reverse Transcriptase Polymerase Chain Reaction
• Signal Transduction (drug effects)
• Survivin
• Time Factors
• Transcription Factor HES-1
• Tumor Stem Cell Assay