Cytogenetic significance of chromosome 17 aberrations and P53 gene mutations as prognostic markers in oral squamous cell carcinoma.

Abstract

BACKGROUND:

Cytogenetic analysis has detected an accumulation of genetic lesions in oral cancers. Numerical changes in chromosome 17 might be associated with an up-regulation of p53 gene, and could contribute to critical events in carcinogenesis. The aim of this study was to reveal possible correlations between the numerical aberrations of chromosome 17, deletion or amplification of the P53 gene and histological grading in patients with oral squamous cell carcinoma (OSCC).

METHODS:

This study was performed retrospectively on anonymous forty paraffin embedded specimens diagnosed with a primary OSCC. Sections were prepared for p53 immunohistochemical staining and FISH technique evaluation.

RESULTS:

All studied cases showed a positive nuclear staining with different indices for the p53 protein. Furthermore, statistical analysis showed a significant difference between all histological types of OSCC. In term of P53 immunoreactivity well differentiated OSCC showed the highest, whereas poorly differentiated showed weakest. Regarding chromosome 17 aberrations and p53 gene mutations, Spearman correlation test revealed a statistical significant positive correlation between chromosome 17 abnormalities and p53 gene mutations as well as with the immunohistochemical expression of p53 proteins. Moreover, the positive association between p53 gene mutations and the expression of p53 protein was statistically significant.

CONCLUSION:

In the light of the previous findings, we concluded that numerical aberrations of chromosome 17 and p53 gene mutations as well as expression of p53 protein have enormous influence on various cellular processes including differentiation and carcinogenesis. Such knowledge provides an easy and simplified approach to prognosis predilection for OSCC.

VIRTUAL SLIDES:

The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/13000_2015_232 .

• Authors: Walid Zedan, Mohamed I Mourad, Sherin M Abd El-Aziz, Nagla M Salamaa, Asem A Shalaby
• Journal: Diagnostic pathology (Diagn Pathol) Vol. 10 Pg. 2 (Feb 22 2015) ISSN: 1746-1596 [Electronic] England
• PMID: 25881131 (Publication Type: Journal Article)

Chemical References

• Biomarkers, Tumor
• TP53 protein, human
• Tumor Suppressor Protein p53

Topics

• Adult
• Biomarkers, Tumor (analysis, genetics)
• Carcinoma, Squamous Cell (chemistry, genetics, pathology)
• Cell Differentiation
• Chromosome Aberrations
• Chromosomes, Human, Pair 17
• DNA Mutational Analysis (methods)
• Female
• Genetic Predisposition to Disease
• Head and Neck Neoplasms (chemistry, genetics, pathology)
• Humans
• Immunohistochemistry
• In Situ Hybridization, Fluorescence
• Male
• Mouth Neoplasms (chemistry, genetics, pathology)
• Mutation
• Neoplasm Grading
• Phenotype
• Predictive Value of Tests
• Retrospective Studies
• Squamous Cell Carcinoma of Head and Neck
• Tumor Suppressor Protein p53 (analysis, genetics)