Abstract |
The aim of the present study was to produce the human dual specificity phosphatase 1 (DUSP1) protein with biological activity and to investigate its in vitro effects on cancer cells. DUSP1 protein was expressed in the baculovirus expression system and purified by Ni-affinity chromatography followed by dialysis in PBS. The purified protein was verified by SDS-PAGE and western blot analysis. Six cancer cell lines were then cultured in the presence of DUSP1 for various periods of time, and the phosphorylated extracellular signal-regulated kinase (p-ERK) content in each cell line was subsequently determined by western blot analysis. Compared to the β-actin level, the amount of p-ERK markedly decreased after 1 h, indicating that DUSP1 suppressed the expression of p-ERK in 6 cancer cell lines examined. Human cervical cancer cells were also collected and counted following co-culture with DUSP1 to examine its effect on the growth rate of cancer cells. A baculovirus expression system for the production of DUSP1 protein was successfully constructed. The p-ERK content was found to be significantly decreased when the cancer cell lines were exposed to DUSP1. The capability of binary fission was reduced when the cells were examined under a microscope. The proliferation of human cervical cancer cells was also inhibited by DUSP1.
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Authors | Peng Cheng, Shuying Zhu, Li Jun, Lihua Huang, Yahui Hong |
Journal | International journal of molecular medicine
(Int J Mol Med)
Vol. 35
Issue 6
Pg. 1715-9
(Jun 2015)
ISSN: 1791-244X [Electronic] Greece |
PMID | 25872469
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Recombinant Proteins
- DUSP1 protein, human
- Dual Specificity Phosphatase 1
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Topics |
- Animals
- Baculoviridae
- Cell Proliferation
(drug effects)
- Dual Specificity Phosphatase 1
(biosynthesis, genetics, pharmacology)
- Gene Expression
- HeLa Cells
- Humans
- MAP Kinase Signaling System
(drug effects)
- Neoplasms
(drug therapy, metabolism, pathology)
- Recombinant Proteins
(biosynthesis, genetics, pharmacology)
- Sf9 Cells
- Spodoptera
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