Abstract |
Fluorescent ubiquitination-based cell-cycle indicator (FUCCI) human cancer (HeLa) cells (red indicates G1; green, S/G2) were exposed to a synchrotron X-ray microbeam. Cells in either G1 or S/G2 were irradiated selectively according to their colour in the same microscopic field. Time-lapse micrographs of the irradiated cells were acquired for 24 h after irradiation. For fluorescent immunostaining, phosphorylated histone proteins (γ-H2AX) indicated the induction of DNA double-strand breaks. The cell cycle was arrested by irradiation at S/G2. In contrast, cells irradiated at G1 progressed to S/G2. The foci were induced in cells irradiated at both G1 and S/G2, suggesting that the G1-S (or S) checkpoint pathway does not function in HeLa cells due to the fact that the cells are functionally p53 deficient, even though X-ray microbeam irradiation significantly induces double-strand breaks. These results demonstrate that single FUCCI cell exposure and live cell imaging are powerful methods for studying the effects of radiation on the cell cycle.
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Authors | A Narita, K Kaminaga, A Yokoya, M Noguchi, K Kobayashi, N Usami, K Fujii |
Journal | Radiation protection dosimetry
(Radiat Prot Dosimetry)
Vol. 166
Issue 1-4
Pg. 192-6
(Sep 2015)
ISSN: 1742-3406 [Electronic] England |
PMID | 25870438
(Publication Type: Journal Article)
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Copyright | © The Author 2015. Published by Oxford University Press. All rights reserved. For Permissions, please email: [email protected]. |
Chemical References |
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Topics |
- Cell Cycle
(radiation effects)
- DNA Breaks, Double-Stranded
(radiation effects)
- Fluorescent Dyes
(analysis)
- HeLa Cells
- Humans
- Image Processing, Computer-Assisted
- Microscopy, Fluorescence
- Mitosis
(radiation effects)
- Phosphorylation
(radiation effects)
- Synchrotrons
- Ubiquitination
(radiation effects)
- X-Rays
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