Abstract | OBJECTIVE: METHODS: Forty male Wistar rats were randomly divided into five groups evenly. Necrotizing pancreatitis was induced in group I and II by retrograde injection of 3% taurocholate into common pancreaticobiliary duct. In group III and IV saline was introduced instead of taurocholate to mimic pressure effect. Infliximab (8mg/kg) was infused through tail vein in group I and III and saline was infused in group II and IV just before laparotomy. Group V underwent sham laparotomy. Serum amylase activity, serum and tissue sialic acid, carbonyl content, malondialdehyde, total antioxidant activity (TAA) and pancreatic histopathology were assessed. RESULTS: In group I serum sialic acid, malondialdehyde, carbonyl content and amylase activity were significantly lower than in group II (p<0.01). There were no significant differences for serum TAA between group I and II (p>0.05). Tissue sialic acid and malondialdehyde in group I were significantly lower than in group II (p<0.01). But tissue TAA in group I was significantly higher than in group II (p<0.01). Carbonyl content of group I was not significantly different from group II (p>0.05). Histopathologically, pancreatic sections of group II demonstrated extensive acinar and fat necrosis, hemorrhage, and inflammation. In group I Infliximab improved histopathological changes (p0.05). CONCLUSION:
|
Authors | S Ozcevik Tekin, S Teksoz, D Terzioglu, A E Arikan, H Ozcevik, E Uslu |
Journal | Bratislavske lekarske listy
(Bratisl Lek Listy)
Vol. 116
Issue 3
Pg. 167-72
( 2015)
ISSN: 0006-9248 [Print] Slovakia |
PMID | 25869565
(Publication Type: Journal Article)
|
Chemical References |
- Anti-Inflammatory Agents, Non-Steroidal
- Antibodies, Monoclonal
- Malondialdehyde
- Taurocholic Acid
- Infliximab
- Amylases
|
Topics |
- Amylases
(metabolism)
- Animals
- Anti-Inflammatory Agents, Non-Steroidal
(administration & dosage)
- Antibodies, Monoclonal
(administration & dosage)
- Edema
(chemically induced, drug therapy)
- Infliximab
- Infusions, Intravenous
- Male
- Malondialdehyde
(metabolism)
- Pancreas
(drug effects, pathology)
- Pancreatitis, Acute Necrotizing
(blood, chemically induced, drug therapy, pathology)
- Random Allocation
- Rats
- Rats, Sprague-Dawley
- Rats, Wistar
- Taurocholic Acid
|