Ameliorative efficacy of tetrahydrocurcumin against arsenic induced oxidative damage, dyslipidemia and hepatic mitochondrial toxicity in rats.

Abstract

Arsenic (As) is a well-known human carcinogen and a potent hepatotoxin. Environmental exposure to arsenic imposes a serious health hazard to humans and other animals worldwide. Tetrahydrocurcumin (THC), one of the major metabolites of curcumin, exhibits many of the same physiological and pharmacological activities as curcumin and in some systems may exert greater antioxidant activity than the curcumin. It has been reported that THC has antioxidant efficacy attributable to the presence of identical β-diketone of 3rd and 5th substitution in heptane moiety. In the present study, rats were orally treated with arsenic alone (5 mg kg(-1) bw/day) with THC (80 mg kg(-1) bw/day) for 28 days. Hepatotoxicity was measured by the increased activities of serum hepatospecific enzymes, namely aspartate transaminase, alanine transaminase, alkaline phosphatase and bilirubin along with increased elevation of lipid peroxidative markers, thiobarbituric acid reactive substances. And also elevated levels of serum cholesterol, triglycerides, free fatty acids and phospholipids were observed in arsenic intoxicated rats. These effects of arsenic were coupled with enhanced mitochondrial swelling, inhibition of cytochrome c oxidase, Ca(2+)ATPase and a decrease in mitochondrial calcium content. The toxic effect of arsenic was also indicated by significantly decreased activities of enzymatic antioxidants such as superoxide dismutase, catalase, and glutathione peroxidase along with non-enzymatic antioxidant such as reduced glutathione. Administration of THC exhibited significant reversal of arsenic induced toxicity in hepatic tissue. All these changes were supported by the reduction of arsenic concentration and histopathological observations of the liver. These results suggest that THC has a protective effect over arsenic induced toxicity in rat.

• Authors: M Muthumani, S Miltonprabu
• Journal: Chemico-biological interactions (Chem Biol Interact) Vol. 235 Pg. 95-105 (Jun 25 2015) ISSN: 1872-7786 [Electronic] Ireland
• PMID: 25869292 (Publication Type: Journal Article)
• Copyright: Copyright © 2015 Elsevier Ireland Ltd. All rights reserved.

Chemical References

• Antioxidants
• Thiobarbituric Acid Reactive Substances
• tetrahydrocurcumin
• Catalase
• Glutathione Peroxidase
• Superoxide Dismutase
• Aspartate Aminotransferases
• Alanine Transaminase
• Glutathione
• Curcumin
• Arsenic

Topics

• Alanine Transaminase (metabolism)
• Animals
• Antioxidants (metabolism)
• Arsenic (adverse effects)
• Arsenic Poisoning (drug therapy, metabolism)
• Aspartate Aminotransferases (metabolism)
• Catalase (metabolism)
• Chemical and Drug Induced Liver Injury (drug therapy, metabolism)
• Curcumin (analogs & derivatives, pharmacology)
• Dyslipidemias (drug therapy, metabolism)
• Glutathione (metabolism)
• Glutathione Peroxidase (metabolism)
• Liver (drug effects, metabolism)
• Male
• Mitochondria (drug effects, metabolism)
• Oxidative Stress (drug effects)
• Rats
• Rats, Wistar
• Superoxide Dismutase (metabolism)
• Thiobarbituric Acid Reactive Substances (metabolism)