Abstract | INTRODUCTION: MATERIAL AND METHODS: LX2 cells were incubated with three different immunosuppressants (rapamycine, mycophenolic acid or cyclosporine A) and co-incubated for 24 and 48 h with apoptotic bodies (AB), produced from Huh7 cells or from Con1 cells (Huh7-cells containing a subgenomic HCV replicon). The engulfment of AB was confirmed by immunofluorescence staining. HSC viability, apoptosis rate and expression of profibrogenic and proapoptotic genes were quantified. RESULTS: In LX2 cells that engulfed Con1 AB, the treatment with mycophenolic acid induced HSC apoptosis and reduced collagen 1alpha 1 expression compared to cylosporine A or rapamycine treatment. In conclusion mycophenolic acid is a potent inducer of HSC apoptosis and attenuates HSC activation and consecutively fibrogenesis in HCV infection. Translational studies will need to confirm these mono-culture results in vivo.
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Authors | Jan Best, Andreas Zahn, Anja Beilfuß, Svenja Sydor, Christian Fingas, Jan-Peter Sowa, Olympia Anastasiou, Vito Cicinnati, Guido Gerken, Ali Canbay, Lars P Bechmann |
Journal | Annals of hepatology
(Ann Hepatol)
2015 May-Jun
Vol. 14
Issue 3
Pg. 396-403
ISSN: 1665-2681 [Print] Mexico |
PMID | 25864221
(Publication Type: Journal Article)
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Chemical References |
- RNA, Viral
- Mycophenolic Acid
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Topics |
- Apoptosis
- Cell Line
- Disease Progression
- Hepacivirus
(genetics)
- Hepatic Stellate Cells
(metabolism, pathology)
- Hepatitis C, Chronic
(metabolism, pathology)
- Humans
- Mycophenolic Acid
(metabolism)
- RNA, Viral
(analysis)
- Real-Time Polymerase Chain Reaction
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