Abstract |
Semaphorin 3A ( Sema3A), originally identified as a potent growth cone collapsing factor in developing sensory neurons, is now recognized as a key player in immune, cardiovascular, bone metabolism and neurological systems. Here we established an anti-Sema3A monoclonal antibody that neutralizes the effects of Sema3A both in vitro and in vivo. The anti-Sema3A neutralization chick IgM antibodies were screened by combining an autonomously diversifying library selection system and an in vitro growth cone collapse assay. We further developed function-blocking chick-mouse chimeric and humanized anti-Sema3A antibodies. We found that our anti-Sema3A antibodies were effective for improving the survival rate in lipopolysaccharide-induced sepsis in mice. Our antibody is a potential therapeutic agent that may prevent the onset of or alleviate symptoms of human diseases associated with Sema3A.
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Authors | Naoya Yamashita, Aoi Jitsuki-Takahashi, Miyuki Ogawara, Wataru Ohkubo, Tomomi Araki, Chie Hotta, Tomohiko Tamura, Shu-ichi Hashimoto, Takashi Yabuki, Toru Tsuji, Yukie Sasakura, Hiromi Okumura, Aki Takaiwa, Chika Koyama, Koji Murakami, Yoshio Goshima |
Journal | International immunology
(Int Immunol)
Vol. 27
Issue 9
Pg. 459-66
(Sep 2015)
ISSN: 1460-2377 [Electronic] England |
PMID | 25855660
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | © The Japanese Society for Immunology. 2015. All rights reserved. For permissions, please e-mail: [email protected]. |
Chemical References |
- Antibodies, Monoclonal
- Antibodies, Neutralizing
- Lipopolysaccharides
- Recombinant Proteins
- Semaphorin-3A
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Topics |
- Animals
- Antibodies, Monoclonal
(immunology)
- Antibodies, Neutralizing
(immunology)
- COS Cells
- Cell Line
- Chickens
- Chlorocebus aethiops
- Humans
- Lipopolysaccharides
(immunology)
- Male
- Mice
- Mice, Inbred C57BL
- Recombinant Proteins
(immunology)
- Semaphorin-3A
(immunology)
- Sepsis
(immunology)
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