[Spectrum-effect relationship of Moutan cortex against lipopolysaccharide-induced acute lung injury].

Abstract

This research is to study the relationship between HPLC fingerprints of Moutan Cortex, Paeoniae Radix Rubra and Paeoniae Radix Alba and their activity on lipopolysaccharide-induced acute lung injury. HPLC fingerprints of each extract of Moutan Cortex,Paeoniae Radix Rubra and Paeoniae Radix Alba were established by an optimized HPLC-MS method. The activities of all samples against protein and tumor necrosis a factor were tested by the model of lipopolysaccharide-induced acute lung injury. The possible relationship between HPLC-MS fingerprints and the activitieswere deduced by the Partial least squares regression analysis method. Samples were analyzed by HPLC-MS/MS to identify the major peaks. The results showed that each sample had some effect on acute lung injury. Four components with a lager contribution rate of efficacy were calculated by the research of spectrum-effect relationship. Moutan Cortex exhibited good activity on acute lung injury, and gallic acid, paeoniflorin, galloylpaeoniflorin and paeonol were the main effective components.

• Authors: Ming-jie Tang, Yong-shan Ye, Qi Zhang, Jian Li, Hai-min Lei, Cheng-ke Cai, Qiang Li
• Journal: Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica (Zhongguo Zhong Yao Za Zhi) Vol. 39 Issue 22 Pg. 4389-93 (Nov 2014) ISSN: 1001-5302 [Print] China
• PMID: 25850273 (Publication Type: Journal Article)

Chemical References

• Acetophenones
• Bridged Bicyclo Compounds, Heterocyclic
• Drugs, Chinese Herbal
• Glucosides
• Lipopolysaccharides
• Monoterpenes
• galloylpaeoniflorin
• moutan cortex
• peoniflorin
• paeonol
• Gallic Acid

Topics

• Acetophenones (chemistry, pharmacology)
• Acute Lung Injury (chemically induced, drug therapy)
• Animals
• Bridged Bicyclo Compounds, Heterocyclic (chemistry, pharmacology)
• Chromatography, High Pressure Liquid (methods)
• Drugs, Chinese Herbal (chemistry, pharmacology)
• Gallic Acid (chemistry, pharmacology)
• Glucosides (chemistry, pharmacology)
• Lipopolysaccharides (pharmacology)
• Male
• Monoterpenes (chemistry, pharmacology)
• Paeonia (chemistry)
• Plant Roots (chemistry)
• Rats
• Rats, Wistar
• Tandem Mass Spectrometry (methods)