Histopathological Analysis of Internal Limiting Membrane Surgically Peeled From Eyes with Epiretinal Membrane.

Abstract

PURPOSE:

To investigate histopathological changes of internal limiting membrane (ILM) in patients with epiretinal membrane (ERM) Materials and Methods: Forty-two eyes of 42 patients who were diagnosed as ERM and enrolled for vitreoretinal surgery were included in this study. Brilliant Blue G (BBG) was used to stain the ILM in all patients. ILM was peeled in all subjects and analyzed by light microscopy (methylene blue-Azur II × 40). ILM samples were then fixed in 2.5% glutaraldehyde solution and examined in JEOL-JEM 1400 and 2100F electron microscope and photographed by CCD camera (Gatan Inc., Pleasanton, CA).

RESULTS:

Remained ERM fragments were observed on 80% of ILM's. Vacuolization of ILM was observed in a patient with diabetic ERM. There were cells and cellular fragments observed mostly at retinal side of ILM which was likely to be a fragment of Muller cells of retina.

CONCLUSIONS:

Most of the ILM's had residual ERM tissue and contained cells and cellular fragments at retinal side of ILM's. ILM peeling might have a role in decreasing ERM recurrence by removal of residual ERM tissues.

• Authors: Özlem Beyazyildiz, Mehmet Hakan Tirhiş, Emine Rümeysa Hekimoğlu, Emrullah Beyazyildiz, Figen Kaymaz, Pelin Yilmazbaş, Faruk Öztürk
• Journal: Current eye research (Curr Eye Res) Vol. 41 Issue 2 Pg. 258-65 ( 2016) ISSN: 1460-2202 [Electronic] England
• PMID: 25848907 (Publication Type: Journal Article, Randomized Controlled Trial, Research Support, Non-U.S. Gov't)

Chemical References

• Fibrillar Collagens
• Indicators and Reagents
• Rosaniline Dyes
• Collagen
• coomassie Brilliant Blue

Topics

• Basement Membrane (ultrastructure)
• Collagen (ultrastructure)
• Endotamponade
• Epiretinal Membrane (etiology, pathology, surgery)
• Fibrillar Collagens (ultrastructure)
• Humans
• Indicators and Reagents
• Microscopy, Electron
• Prospective Studies
• Rosaniline Dyes
• Staining and Labeling (methods)
• Tomography, Optical Coherence
• Visual Acuity (physiology)
• Vitreoretinal Surgery