Characterization of the thymus leukemia (TL) product encoded by the BALB/c T3c gene by DNA-mediated gene transfer. Comparison to the T13c product and BALB/c leukemia TL.

Abstract

Using DNA-mediated gene transfer, we have studied the TL protein products encoded by both the T3c and T13c BALB/c genes. Biochemically, the proteins differed in their m.w. and pI points; serologically, although both molecules were recognized by TL alloantiserum, only the T13c protein was recognized by monoclonal TL antibodies. Interestingly, both proteins were serologically and immunochemically recognized by leukemia-specific TL.4 antiserum. The quantity of cell surface T13 was significantly greater than T3 possibly due to the less efficient splicing of T3 transcripts in the L cell nucleus; both genes directed the synthesis of cytoplasmic RNA containing an unspliced intron 3 as assessed by S1 analysis. In toto, the results suggest that T3c is similar or perhaps identical with the novel TL product previously identified on the surface of certain x-ray-induced BALB/c leukemias.

• Authors: M J Chorney, H Mashimo, Y Bushkin, S G Nathenson
• Journal: Journal of immunology (Baltimore, Md. : 1950) (J Immunol) Vol. 143 Issue 11 Pg. 3762-8 (Dec 01 1989) ISSN: 0022-1767 [Print] United States
• PMID: 2584718 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• DNA, Neoplasm
• Membrane Glycoproteins
• Neoplasm Proteins
• thymus-leukemia antigens

Topics

• Animals
• Cloning, Molecular
• DNA, Neoplasm (physiology)
• Enzyme-Linked Immunosorbent Assay
• Genes, Neoplasm
• Leukemia, T-Cell (genetics, immunology)
• Membrane Glycoproteins (genetics, isolation & purification)
• Mice
• Mice, Inbred BALB C
• Neoplasm Proteins (genetics, isolation & purification)
• Phenotype
• Precipitin Tests
• Transfection