Ex vivo uranium decontamination efficiency on wounded skin and in vitro skin toxicity of a calixarene-loaded nanoemulsion.

Abstract

The present work aims at studying the decontamination efficacy of a calixarene-loaded nanoemulsion on two ex vivo wounded skin models mimicking superficial stings or cuts contaminated with uranium, and on a third model using excoriation. The decontaminating formulation was compared with the currently used radio-decontaminating soapy water (Trait rouge®) treatment. Moreover, to assess skin damage potentially induced by the undiluted nanoemulsion, in vitro toxicity studies were conducted on an in vitro reconstructed human epidermis, coupled with three different toxicity tests [3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyl tetrazolium bromide, lactate dehydrogenase, and interleukin-1-α]. This work demonstrated not only a significant decontamination activity of the calixarene nanoemulsion on wounded skin, ranging from 92% to 94% of the applied uranium solution according to the ex vivo model used, but also the absence of side effects of this promising treatment.

• Authors: Sophie Grives, Guillaume Phan, Guillaume Morat, David Suhard, Francois Rebiere, Elias Fattal
• Journal: Journal of pharmaceutical sciences (J Pharm Sci) Vol. 104 Issue 6 Pg. 2008-2017 (Jun 2015) ISSN: 1520-6017 [Electronic] United States
• PMID: 25846633 (Publication Type: Journal Article)
• Copyright: © 2015 Wiley Periodicals, Inc. and the American Pharmacists Association.

Chemical References

• Emulsions
• Phenols
• calix(6)arene
• Calixarenes
• Uranium

Topics

• Animals
• Calixarenes (chemistry, pharmacology, toxicity)
• Decontamination (methods)
• Emulsions (chemistry, pharmacology, toxicity)
• Humans
• Phenols (chemistry, pharmacology, toxicity)
• Skin (drug effects, pathology, ultrastructure)
• Swine
• Uranium (isolation & purification)
• Wound Healing (drug effects)